AB198286

Anti-MTCO2 antibody

Name: Anti-MTCO2 antibody (ab198286) | Abcam
Brand: Abcam Limited
SKU: AB198286
Price: 535 USD
Availability: InStock

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(18 Publications)

Rabbit Polyclonal MTCO2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human MT-CO2.

View Alternative Names

COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody (AB198286)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody (AB198286)

Immunohistochemical analysis of paraffin embedded Human skin tissue labeling MTCO2 with ab198286 at 1/5 dilution dilution.

Supplier Data

Western blot - Anti-MTCO2 antibody (AB198286)

10% SDS-PAGE

All lanes:

Western blot - Anti-MTCO2 antibody (ab198286) at 1/150 dilution

All lanes:

Mouse heart tissue lysate at 30 µg

Predicted band size: 25 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MT-CO2. The exact immunogen used to generate this antibody is proprietary information.

P00403

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5 - 1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/150 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'MTCO2' also known as 'mt-co2' or 'mtco2e' is a mitochondrial gene that encodes for a component of the cytochrome c oxidase complex referred to as Complex IV in the electron transport chain. The protein plays a mechanical role in facilitating electron transfer within mitochondria an essential process in cellular respiration. MTCO2 is predominantly expressed in tissues with high energy demands such as muscle and neurons. The known mass of the MTCO2 protein is approximately 25 kDa. It sits in the mitochondrial inner membrane where it contributes to creating the proton gradient driving ATP synthesis.

Biological function summary

MTCO2 (or cytochrome c oxidase subunit II) serves as an important player in aerobic respiration. It is part of the cytochrome c oxidase complex which forms the last enzyme complex of the electron transport chain. As part of this complex MTCO2 facilitates the transfer of electrons from cytochrome c to oxygen resulting in the reduction of oxygen to water. This electron transfer is paired with proton translocation across the mitochondrial membrane which is critical for ATP production.

Pathways

MTCO2 contributes significantly to the oxidative phosphorylation pathway which is essential for ATP production in eukaryotic cells. It directly interacts with other components of the mitochondrial electron transport chain like cytochrome c and NADH dehydrogenase which are critical for maintaining the flow of electrons and the integrity of the energy production process. Another pathway it is part of is the apoptosis pathway regulated by non-lethal stress conditions where controlled release of cytochrome c can trigger programmed cell death.

MTCO2 mutations and dysfunctions have been linked with mitochondrial disorders especially those affecting energy-demanding tissues leading to conditions such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These disorders result from compromised oxidative phosphorylation leading to inadequate energy supply. The dysfunction of cytochrome c oxidase which contains the MTCO2 subunit is a central aspect of these diseases often tying this protein to other complexes within the electron transport chain that also underpin mitochondrial diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information MT-CO2

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5261 PubMed40480980

2025

TANGO2 binds crystallin alpha B and its loss causes desminopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Stentenbach,Laetitia A Hughes,Samuel V Fagan,Blake Payne,Danielle L Rudler,Stefan J Siira,Tim McCubbin,Anaëlle Chopin,Kara L Perks,Judith A Ermer,James Hendry,Teagan S Er,Shanti Balasubramaniam,Joel A Eliades,Livia C Hool,Nicolle H Packer,Edward S X Moh,Benjamin S Padman,Oliver Rackham,Aleksandra Filipovska

Investigational new drugs 43:669-678 PubMed40404898

2025

Peptide YY fragment PYY1-36 disrupts mitochondrial biogenesis via RBM43-dependent PGC-1α translation inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Benkun Liu,Fucheng Zhou,Bowen Shi,Yubo Yan,Yanbo Wang,Junfeng Wang,Yaoguo Lang,Shidong Xu

Theranostics 15:4890-4908 PubMed40303347

2025

Pharmacological targeting of mitophagy via ALT001 improves herpes simplex virus 1 (HSV1)-mediated microglial inflammation and promotes amyloid β phagocytosis by restricting HSV1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Soo-Jin Oh,Young Yeon Kim,Ruiying Ma,Seok Tae Choi,Se Myeong Choi,Jong Hyun Cho,Ji-Yeun Hur,Yongjin Yoo,Kihoon Han,Hosun Park,Jeanho Yun,Ok Sarah Shin

Nature communications 16:212 PubMed39747079

2025

Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yi,Guoqiang Wang,Wenhua Zhang,Shuhan Yu,Junjie Fei,Tingting An,Jianqiao Yi,Fengtian Li,Ting Huang,Jian Yang,Mengmeng Niu,Yang Wang,Chuan Xu,Zhi-Xiong Jim Xiao

Cell biology and toxicology 41:7 PubMed39707117

2024

METTL3, m6A modification, and EGR1: interplay affecting myocardial I/R injury outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Xun Zhang,Shi-Xiong Wu,Qing Chang,Zhi-Kun Zheng,Jing Xu

Theranostics 14:56-74 PubMed38164158

2024

Selective induction of Rab9-dependent alternative mitophagy using a synthetic derivative of isoquinoline alleviates mitochondrial dysfunction and cognitive deficits in Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Jee-Hyun Um,Dong Jin Shin,Se Myeong Choi,Alen Benhur Pravin Nathan,Young Yeon Kim,Da Ye Lee,Dae Jin Jeong,Dong Hyun Kim,Kyung Hwa Kim,Young Hye Kim,Jihoon Nah,Jeong-Hee Jeong,Eunhee Yoo,Hwa Kyoung Shin,Hwan Tae Park,Jihoon Jo,Jong Hyun Cho,Jeanho Yun

Scientific reports 13:15339 PubMed37714940

2023

Remdesivir increases mtDNA copy number causing mild alterations to oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole DeFoor,Swagatika Paul,Shuang Li,Erwin K Gudenschwager Basso,Valentina Stevenson,Jack L Browning,Anna K Prater,Samantha Brindley,Ge Tao,Alicia M Pickrell

Cell & bioscience 12:103 PubMed35794642

2022

Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Xihui Chen,Fangfang Liu,Bowen Li,Yufeng Wang,Lijuan Yuan,Anan Yin,Qi Chen,Weihong Hu,Yan Yao,Mengjie Zhang,YuanMing Wu,Kun Chen

PLoS genetics 18:e1010138 PubMed35404932

2022

Tumor suppressor PALB2 maintains redox and mitochondrial homeostasis in the brain and cooperates with ATG7/autophagy to suppress neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yanying Huo,Akshada Sawant,Yongmei Tan,Amar H Mahdi,Tao Li,Hui Ma,Vrushank Bhatt,Run Yan,Jake Coleman,Cheryl F Dreyfus,Jessie Yanxiang Guo,M Maral Mouradian,Eileen White,Bing Xia

Human molecular genetics 31:3597-3612 PubMed35147173

2022

Deleterious variants in CRLS1 lead to cardiolipin deficiency and cause an autosomal recessive multi-system mitochondrial disease.

Applications

Unspecified application

Species

Unspecified reactive species

Richard G Lee,Shanti Balasubramaniam,Maike Stentenbach,Tom Kralj,Tim McCubbin,Benjamin Padman,Janine Smith,Lisa G Riley,Archana Priyadarshi,Liuyu Peng,Madison R Nuske,Richard Webster,Ken Peacock,Philip Roberts,Zornitza Stark,Gabrielle Lemire,Yoko A Ito,Kym M Boycott,Michael T Geraghty,Jan Bert van Klinken,Sacha Ferdinandusse,Ying Zhou,Rebecca Walsh,Esteban Marcellin,David R Thorburn,Tony Rosciolli,Janice Fletcher,Oliver Rackham,Frédéric M Vaz,Gavin E Reid,Aleksandra Filipovska

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MTCO2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody (AB198286)

Western blot - Anti-MTCO2 antibody (AB198286)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise