AB91317

Anti-MTCO2 antibody

Name: Anti-MTCO2 antibody (ab91317) | Abcam
Brand: Abcam Limited
SKU: AB91317
Price: 225 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(19 Publications)

Rabbit Polyclonal MTCO2 antibody. Suitable for WB and reacts with Human samples. Cited in 19 publications.

View Alternative Names

COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II

1 Images

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Western blot - Anti-MTCO2 antibody (AB91317)

All lanes:

Western blot - Anti-MTCO2 antibody (ab91317) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 3:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 4:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 5:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 25 kDa

Observed band size: 120 kDa,15 kDa,21 kDa,65 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'MTCO2' also known as 'mt-co2' or 'mtco2e' is a mitochondrial gene that encodes for a component of the cytochrome c oxidase complex referred to as Complex IV in the electron transport chain. The protein plays a mechanical role in facilitating electron transfer within mitochondria an essential process in cellular respiration. MTCO2 is predominantly expressed in tissues with high energy demands such as muscle and neurons. The known mass of the MTCO2 protein is approximately 25 kDa. It sits in the mitochondrial inner membrane where it contributes to creating the proton gradient driving ATP synthesis.

Biological function summary

MTCO2 (or cytochrome c oxidase subunit II) serves as an important player in aerobic respiration. It is part of the cytochrome c oxidase complex which forms the last enzyme complex of the electron transport chain. As part of this complex MTCO2 facilitates the transfer of electrons from cytochrome c to oxygen resulting in the reduction of oxygen to water. This electron transfer is paired with proton translocation across the mitochondrial membrane which is critical for ATP production.

Pathways

MTCO2 contributes significantly to the oxidative phosphorylation pathway which is essential for ATP production in eukaryotic cells. It directly interacts with other components of the mitochondrial electron transport chain like cytochrome c and NADH dehydrogenase which are critical for maintaining the flow of electrons and the integrity of the energy production process. Another pathway it is part of is the apoptosis pathway regulated by non-lethal stress conditions where controlled release of cytochrome c can trigger programmed cell death.

MTCO2 mutations and dysfunctions have been linked with mitochondrial disorders especially those affecting energy-demanding tissues leading to conditions such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These disorders result from compromised oxidative phosphorylation leading to inadequate energy supply. The dysfunction of cytochrome c oxidase which contains the MTCO2 subunit is a central aspect of these diseases often tying this protein to other complexes within the electron transport chain that also underpin mitochondrial diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information MT-CO2

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:193-210 PubMed39567835

2024

Pathogenic PDE12 variants impair mitochondrial RNA processing causing neonatal mitochondrial disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey Van Haute,Petra Páleníková,Jia Xin Tang,Pavel A Nash,Mariella T Simon,Angela Pyle,Monika Oláhová,Christopher A Powell,Pedro Rebelo-Guiomar,Alexander Stover,Michael Champion,Charulata Deshpande,Emma L Baple,Karen L Stals,Sian Ellard,Olivia Anselem,Clémence Molac,Giulia Petrilli,Laurence Loeuillet,Sarah Grotto,Tania Attie-Bitach,Jose E Abdenur,Robert W Taylor,Michal Minczuk

Journal of translational medicine 22:419 PubMed38702818

2024

VEGFR2 blockade inhibits glioblastoma cell proliferation by enhancing mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Min Guo,Junhao Zhang,Jiang Han,Yingyue Hu,Hao Ni,Juan Yuan,Yang Sun,Meijuan Liu,Lifen Gao,Wangjun Liao,Chunhong Ma,Yaou Liu,Shuijie Li,Nailin Li

Genetics in medicine : official journal of the American College of Medical Genetics 26:101117 PubMed38459834

2024

Alternative splicing expands the clinical spectrum of NDUFS6-related mitochondrial disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Camila Armirola-Ricaurte,Noortje Zonnekein,Georgios Koutsis,Silvia Amor-Barris,Ana Lara Pelayo-Negro,Derek Atkinson,Stephanie Efthymiou,Valentina Turchetti,Argyris Dinopoulos,Antonio Garcia,Mert Karakaya,German Moris,Ayşe Ipek Polat,Uluç Yiş,Carmen Espinos,Liedewei Van de Vondel,Els De Vriendt,Georgia Karadima,Brunhilde Wirth,Michael Hanna,Henry Houlden,Jose Berciano,Albena Jordanova

International journal of molecular sciences 24: PubMed37298383

2023

The C-Terminal Tail of Mitochondrial Transcription Factor A Is Dispensable for Mitochondrial DNA Replication and Transcription In Situ.

Applications

Unspecified application

Species

Unspecified reactive species

Natalya Kozhukhar,Mikhail F Alexeyev

Cells 11: PubMed36497015

2022

TFAM's Contributions to mtDNA Replication and OXPHOS Biogenesis Are Genetically Separable.

Applications

Unspecified application

Species

Unspecified reactive species

Natalya Kozhukhar,Mikhail F Alexeyev

Cells 11: PubMed35883613

2022

A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Natalya Kozhukhar,Domenico Spadafora,Yelitza A R Rodriguez,Mikhail F Alexeyev

Nature 607:593-603 PubMed35768510

2022

Mitochondrial RNA modifications shape metabolic plasticity in metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvain Delaunay,Gloria Pascual,Bohai Feng,Kevin Klann,Mikaela Behm,Agnes Hotz-Wagenblatt,Karsten Richter,Karim Zaoui,Esther Herpel,Christian Münch,Sabine Dietmann,Jochen Hess,Salvador Aznar Benitah,Michaela Frye

Nature metabolism 4:739-758 PubMed35760869

2022

Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shuijie Li,Wenyu Li,Juan Yuan,Petra Bullova,Jieyu Wu,Xuepei Zhang,Yong Liu,Monika Plescher,Javier Rodriguez,Oscar C Bedoya-Reina,Paulo R Jannig,Paula Valente-Silva,Meng Yu,Marie Arsenian Henriksson,Roman A Zubarev,Anna Smed-Sörensen,Carolyn K Suzuki,Jorge L Ruas,Johan Holmberg,Catharina Larsson,C Christofer Juhlin,Alex von Kriegsheim,Yihai Cao,Susanne Schlisio

Journal of materials chemistry. B 8:10825-10836 PubMed33174901

2020

Intracellular delivery of therapeutic antisense oligonucleotides targeting mRNA coding mitochondrial proteins by cell-penetrating peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Carmine Pasquale Cerrato,Tove Kivijärvi,Roberta Tozzi,Tõnis Lehto,Maxime Gestin,Ülo Langel

Frontiers in genetics 10:1393 PubMed32082360

2020

RNase H1 Regulates Mitochondrial Transcription and Translation the Degradation of 7S RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Joanna Rusecka,Katarzyna Tońska,Massimo Zeviani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MTCO2 antibody

Western blot - Anti-MTCO2 antibody (AB91317)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise