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AB247921

Anti-MTCO2 antibody [EPR3313] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MTCO2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)

This data was developed using ab109739, the same antibody clone in a different buffer formulation.

ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)

This data was developed using ab109739, the same antibody clone in a different buffer formulation.

ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)

This data was developed using ab109739, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Cytochrome C oxidase subunit II with ab109739 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution.

Confocal image showing mitochondria staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain COX IV at 1/1000 dilution (Magenta).

The negative controls are as follows :
-ve control 1 : ab109739 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.
-ve control 2 : anti-COX IV mouse monoclonal antibody - Mitochondrial Marker antibody at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary at 1/1000 dilution.

Western blot - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)
  • WB

Unknown

Western blot - Anti-MTCO2 antibody [EPR3313] - BSA and Azide free (AB247921)

This data was developed using ab109739, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MTCO2 antibody [EPR3313] (<a href='/en-us/products/primary-antibodies/mtco2-antibody-epr3313-ab109739'>ab109739</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

THP1 cell lysate at 10 µg

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3313

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended. Heat up to 98 degree C, below boiling, and then let cool for 10-20 min.</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab247921 is the carrier-free version of ab109739.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'MTCO2' also known as 'mt-co2' or 'mtco2e' is a mitochondrial gene that encodes for a component of the cytochrome c oxidase complex referred to as Complex IV in the electron transport chain. The protein plays a mechanical role in facilitating electron transfer within mitochondria an essential process in cellular respiration. MTCO2 is predominantly expressed in tissues with high energy demands such as muscle and neurons. The known mass of the MTCO2 protein is approximately 25 kDa. It sits in the mitochondrial inner membrane where it contributes to creating the proton gradient driving ATP synthesis.
Biological function summary

MTCO2 (or cytochrome c oxidase subunit II) serves as an important player in aerobic respiration. It is part of the cytochrome c oxidase complex which forms the last enzyme complex of the electron transport chain. As part of this complex MTCO2 facilitates the transfer of electrons from cytochrome c to oxygen resulting in the reduction of oxygen to water. This electron transfer is paired with proton translocation across the mitochondrial membrane which is critical for ATP production.

Pathways

MTCO2 contributes significantly to the oxidative phosphorylation pathway which is essential for ATP production in eukaryotic cells. It directly interacts with other components of the mitochondrial electron transport chain like cytochrome c and NADH dehydrogenase which are critical for maintaining the flow of electrons and the integrity of the energy production process. Another pathway it is part of is the apoptosis pathway regulated by non-lethal stress conditions where controlled release of cytochrome c can trigger programmed cell death.

MTCO2 mutations and dysfunctions have been linked with mitochondrial disorders especially those affecting energy-demanding tissues leading to conditions such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These disorders result from compromised oxidative phosphorylation leading to inadequate energy supply. The dysfunction of cytochrome c oxidase which contains the MTCO2 subunit is a central aspect of these diseases often tying this protein to other complexes within the electron transport chain that also underpin mitochondrial diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information MT-CO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 136:2003-2012 PubMed27349864

2016

Thyroid Hormones Enhance Mitochondrial Function in Human Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Vidali,Jérémy Chéret,Melanie Giesen,Swantje Haeger,Majid Alam,Rachel E B Watson,Abigail K Langton,Matthias Klinger,Jana Knuever,Wolfgang Funk,Barbara Kofler,Ralf Paus
View all publications
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