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Rabbit Recombinant Monoclonal MTCO2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (AB239889), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (AB239889), expandable thumbnail
  • Immunoprecipitation - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (AB239889), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (AB239889), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Expected
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

6 products for Alternative Product

2 products for Alternative Version

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II

Recommended products

Rabbit Recombinant Monoclonal MTCO2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3314
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab239889 is the carrier-free version of Anti-MTCO2 antibody [EPR3314] ab79393.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

'MTCO2' also known as 'mt-co2' or 'mtco2e' is a mitochondrial gene that encodes for a component of the cytochrome c oxidase complex referred to as Complex IV in the electron transport chain. The protein plays a mechanical role in facilitating electron transfer within mitochondria an essential process in cellular respiration. MTCO2 is predominantly expressed in tissues with high energy demands such as muscle and neurons. The known mass of the MTCO2 protein is approximately 25 kDa. It sits in the mitochondrial inner membrane where it contributes to creating the proton gradient driving ATP synthesis.

Biological function summary

MTCO2 (or cytochrome c oxidase subunit II) serves as an important player in aerobic respiration. It is part of the cytochrome c oxidase complex which forms the last enzyme complex of the electron transport chain. As part of this complex MTCO2 facilitates the transfer of electrons from cytochrome c to oxygen resulting in the reduction of oxygen to water. This electron transfer is paired with proton translocation across the mitochondrial membrane which is critical for ATP production.

Pathways

MTCO2 contributes significantly to the oxidative phosphorylation pathway which is essential for ATP production in eukaryotic cells. It directly interacts with other components of the mitochondrial electron transport chain like cytochrome c and NADH dehydrogenase which are critical for maintaining the flow of electrons and the integrity of the energy production process. Another pathway it is part of is the apoptosis pathway regulated by non-lethal stress conditions where controlled release of cytochrome c can trigger programmed cell death.

Associated diseases and disorders

MTCO2 mutations and dysfunctions have been linked with mitochondrial disorders especially those affecting energy-demanding tissues leading to conditions such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These disorders result from compromised oxidative phosphorylation leading to inadequate energy supply. The dysfunction of cytochrome c oxidase which contains the MTCO2 subunit is a central aspect of these diseases often tying this protein to other complexes within the electron transport chain that also underpin mitochondrial diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889)

    Anti-MTCO2 antibody [EPR3314] ab79393 at 1/100 dilution staining Cytochrome C oxidase subunit II in human liver (A), human heart (B) and HeLa cell line (C) by Immunohistochemistry, Paraffin-embedded tissues. Detection method of A and B was HRP-conjugated anti-rabbit with DAB substrate used for staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTCO2 antibody [EPR3314] ab79393).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889)

    ICC/IF image of Anti-MTCO2 antibody [EPR3314] ab79393 stained HepG2 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-MTCO2 antibody [EPR3314] ab79393, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTCO2 antibody [EPR3314] ab79393).

  • Immunoprecipitation - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889), expandable thumbnail

    Immunoprecipitation - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889)

    This data was developed using Anti-MTCO2 antibody [EPR3314] ab79393, the same antibody clone in a different buffer formulation.
    Purified Anti-MTCO2 antibody [EPR3314] ab79393 at 1/50 dilution (2μg) immunoprecipitating MTCO2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-MTCO2 antibody [EPR3314] ab79393 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MTCO2 antibody [EPR3314] ab79393 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 21 kDa

    All lanes: Immunoprecipitation - Anti-MTCO2 antibody [EPR3314] (Anti-MTCO2 antibody [EPR3314] ab79393)

    Predicted band size: 25 kDa

  • Flow Cytometry (Intracellular) - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MTCO2 antibody [EPR3314] - BSA and Azide free (ab239889)

    Overlay histogram showing HepG2 cells stained with Anti-MTCO2 antibody [EPR3314] ab79393 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-MTCO2 antibody [EPR3314] ab79393, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTCO2 antibody [EPR3314] ab79393).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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