AB156031

Anti-MTCO2 antibody [MM7D-5A11CA9]

Name: Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031) | Abcam
Brand: Abcam Limited
SKU: AB156031

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(5 Publications)

Mouse Monoclonal MTCO2 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with African green monkey, Human samples. Cited in 5 publications.

View Alternative Names

COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Immunocytochemical analysis of 143B (Human osteosarcoma) wild type (WT) and mtDNA lacking (p0) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red) and anti-HSP60 (ab46798) at 1/1000 (green) as a counter stain. Secondary : Alexa 594 goat anti-mouse IgG at 1/1000 and Alexa 488 goat anti-rat IgG at 1/1000. ab156031 shows good reactivity with the wild type (143B-WT) cell line, but shows no signal with the mtDNA lacking (143B-p0) cell line. Cytochrome c oxidase 2 is exclusively encoded by mitochondrial DNA. The anti-HSP60 counter stain antibody identified mitochondria in both cell lines.

Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Flow Cyt

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Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labeling COXII with ab156031 (blue) at 1µg/ml and an equal amount of an isotype control antibody (red).

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Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Immunoprecipitation Coomassie stained gel for Anti-COXII antibody (ab156031).
Lane 1 : Ladder
Lane 2 : COXII Immunoprecipitated from 1mg Human Heart Homogenate (HHH) Extract. ab156031 anti-COXII captures the entire Cytochrome c oxidase complex in immunoprecipitation.

All lanes:

Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)

Predicted band size: 25 kDa

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Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

1X Blocking Buffer (ab126587) in H₂O was used for blocking and incubation steps.

All lanes:

Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031) at 5 µg/mL

Lane 1:

Human Liver Mitochondria Extract at 40 µg

Lane 2:

Human Heart Mitochondria Extrac at 40 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

Predicted band size: 25 kDa

false

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Immunocytochemical analysis of COS7 (monkey kidney fibroblast) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red). Secondary : Alexa 594 goat anti-mouse IgG at 1/1000. ab156031 shows good reactivity with the COS7 cell type indicating cross reactivity with monkey cytochrome c oxidase.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM7D-5A11CA9

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

ICC/IF, IP, Flow Cyt, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

Near homogeneity as judged by SDS-PAGE. ab156031 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'MTCO2' also known as 'mt-co2' or 'mtco2e' is a mitochondrial gene that encodes for a component of the cytochrome c oxidase complex referred to as Complex IV in the electron transport chain. The protein plays a mechanical role in facilitating electron transfer within mitochondria an essential process in cellular respiration. MTCO2 is predominantly expressed in tissues with high energy demands such as muscle and neurons. The known mass of the MTCO2 protein is approximately 25 kDa. It sits in the mitochondrial inner membrane where it contributes to creating the proton gradient driving ATP synthesis.

Biological function summary

MTCO2 (or cytochrome c oxidase subunit II) serves as an important player in aerobic respiration. It is part of the cytochrome c oxidase complex which forms the last enzyme complex of the electron transport chain. As part of this complex MTCO2 facilitates the transfer of electrons from cytochrome c to oxygen resulting in the reduction of oxygen to water. This electron transfer is paired with proton translocation across the mitochondrial membrane which is critical for ATP production.

Pathways

MTCO2 contributes significantly to the oxidative phosphorylation pathway which is essential for ATP production in eukaryotic cells. It directly interacts with other components of the mitochondrial electron transport chain like cytochrome c and NADH dehydrogenase which are critical for maintaining the flow of electrons and the integrity of the energy production process. Another pathway it is part of is the apoptosis pathway regulated by non-lethal stress conditions where controlled release of cytochrome c can trigger programmed cell death.

MTCO2 mutations and dysfunctions have been linked with mitochondrial disorders especially those affecting energy-demanding tissues leading to conditions such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These disorders result from compromised oxidative phosphorylation leading to inadequate energy supply. The dysfunction of cytochrome c oxidase which contains the MTCO2 subunit is a central aspect of these diseases often tying this protein to other complexes within the electron transport chain that also underpin mitochondrial diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information MT-CO2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:1449-1460 PubMed32016470

2020

Sialylation of FGFR1 by ST6Gal‑I overexpression contributes to ovarian cancer cell migration and chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Ou,Xiuzhen He,Naihua Liu,Yuwei Song,Jinyuan Li,Lvfen Gao,Xinke Huang,Zhendong Deng,Xiaoyu Wang,Shaoqiang Lin

Cell death & disease 10:940 PubMed31819039

2019

Intramitochondrial Src kinase links mitochondrial dysfunctions and aggressiveness of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Ange Djeungoue-Petga,Olivier Lurette,Stéphanie Jean,Geneviève Hamel-Côté,Rebeca Martín-Jiménez,Marine Bou,Astrid Cannich,Patrick Roy,Etienne Hebert-Chatelain

Archives of pharmacal research 42:1052-1062 PubMed31802425

2019

Involvement of mitochondrial biogenesis during the differentiation of human periosteum-derived mesenchymal stem cells into adipocytes, chondrocytes and osteocytes.

Applications

Unspecified application

Species

Unspecified reactive species

A Ram Lee,Dong Kyu Moon,Adrian Siregar,Sun Young Moon,Ryoung-Hoon Jeon,Young-Bum Son,Bo Gyu Kim,Young-Sool Hah,Sun-Chul Hwang,June-Ho Byun,Dong Kyun Woo

American journal of physiology. Cell physiology 316:C862-C875 PubMed30865517

2019

Acute increases in -GlcNAc indirectly impair mitochondrial bioenergetics through dysregulation of LonP1-mediated mitochondrial protein complex turnover.

Applications

Unspecified application

Species

Unspecified reactive species

JaLessa N Wright,Gloria A Benavides,Michelle S Johnson,Willayat Wani,Xiaosen Ouyang,Luyun Zou,Helen E Collins,Jianhua Zhang,Victor Darley-Usmar,John C Chatham

International journal of environmental research an 16: PubMed30813396

2019

Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Chiara Lionetti,Federico Mutti,Erica Soldati,Maria Rita Fumagalli,Valentina Coccé,Graziano Colombo,Emanuela Astori,Alessandro Miani,Aldo Milzani,Isabella Dalle-Donne,Emilio Ciusani,Giulio Costantini,Caterina A M La Porta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTCO2 antibody [MM7D-5A11CA9]

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (AB156031)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise