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AB110259

Anti-MTCO3 antibody [DA5BC4]

3

(3 Reviews)

|

(49 Publications)

Mouse Monoclonal MTCO3 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae, Rat, Human, Mouse samples. Cited in 49 publications.

View Alternative Names

COIII, COXIII, MTCO3, MT-CO3, Cytochrome c oxidase subunit 3, Cytochrome c oxidase polypeptide III

1 Images
Western blot - Anti-MTCO3 antibody [DA5BC4] (AB110259)
  • WB

Unknown

Western blot - Anti-MTCO3 antibody [DA5BC4] (AB110259)

All lanes:

Western blot - Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 5 µg

Lane 2:

Isolated mitochondria Rat heart at 10 µg

Lane 3:

Isolated mitochondria from Mouse heart at 10 µg

Predicted band size: 30 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DA5BC4

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Human, Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
ab110259 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Near homogeneity ab110259 was judged as near homogeneity by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTCO3 also known as Cytochrome c oxidase subunit 3 is a protein component essential to mitochondrial function. It weighs approximately 30 kDa and plays a structural role in Cytochrome c oxidase which is part of the mitochondrial electron transport chain. Expression of MTCO3 occurs in the mitochondria of cells with high energy demand including muscle and heart tissue. This protein forms part of a larger complex that helps facilitate the transfer of electrons from cytochrome c to oxygen a critical step in cellular respiration.
Biological function summary

MTCO3 interacts with other subunits within the Cytochrome c oxidase complex to ensure efficient electron transfer and reduction of oxygen to water. This enzyme complex is fundamental in maintaining the proton gradient across the mitochondrial membrane which drives ATP synthesis. As part of this complex MTCO3 helps in coupling electron transfer with proton translocation therefore playing an important role in energy production within cells.

Pathways

MTCO3 is integral to the oxidative phosphorylation pathway which is an important process in cellular energy production. This pathway enables ATP generation through the mitochondrial electron transport chain critical for cell survival and function. MTCO3 collaborates with proteins like MTCO1 and MTCO2 which also belong to the Cytochrome c oxidase complex in these biological processes.

MTCO3 relates to conditions such as mitochondrial myopathy and Leigh syndrome. These are genetic disorders affecting mitochondrial energy production often due to mutations affecting components of the electron transport chain. MTCO3 has connections to these diseases through its interaction with other electron transport chain constituents such as MTND1 and ATP6 which are also implicated in mitochondrial disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information MT-CO3

Publications (49)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:8063-8083 PubMed40626139

2025

Macrophage Membrane-Coated Liposomes Delivering Vonoprazan Disrupt Mitochondrial Oxidative Phosphorylation in Diffuse Large B-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Wang,Lei Wang,Qingqing Fan,Jingyan Xu,Yifan Li,Jiale Xu,Bei Zhao,Dan Ge,Longying Xiong,Yanping Wu,Yun Zhu,Min Chen

Aging and disease 16:3638-3648 PubMed39965253

2025

Targeting Mitochondrial Integrity as a New Senolytic Strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Eliska Vacurova,Edita Vlachova,Jan Stursa,Klara Bohacova,Tereza Havrlantova,Vojtech Skop,Barbora Judita Kasperova,Lukas Werner,Jiri Neuzil,Martin Haluzik,Sona Stemberkova Hubackova

Frontiers in genetics 14:1278572 PubMed38098475

2023

Prominent muscle involvement in a familial form of mitochondrial disease due to a variant.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Rimoldi,Francesca Magri,Sara Antognozzi,Michela Ripolone,Sabrina Salani,Daniela Piga,Letizia Bertolasi,Simona Zanotti,Patrizia Ciscato,Francesco Fortunato,Maurizio Moggio,Stefania Corti,Giacomo Pietro Comi,Dario Ronchi

Archives of biochemistry and biophysics 744:109665 PubMed37348627

2023

Evidence that the catalytic mechanism of heme a synthase involves the formation of a carbocation stabilized by a conserved glutamate.

Applications

Unspecified application

Species

Unspecified reactive species

Elise D Rivett,Hannah G Addis,Jonathan V Dietz,Jayda A Carroll-Deaton,Shipra Gupta,Koji L Foreman,Minh Anh Dang,Jennifer L Fox,Oleh Khalimonchuk,Eric L Hegg

The Journal of biological chemistry 299:103018 PubMed36796514

2023

Mitochondrial respiration reduces exposure of the nucleus to oxygen.

Applications

Unspecified application

Species

Unspecified reactive species

Mateus Prates Mori,Rozhin Penjweini,Jin Ma,Greg Alspaugh,Alessio Andreoni,Young-Chae Kim,Ping-Yuan Wang,Jay R Knutson,Paul M Hwang

Journal of cell science 135: PubMed36185004

2022

Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.

Applications

Unspecified application

Species

Unspecified reactive species

Antoineen J White,Clare S Harper,Erica M Rosario,Jonathan V Dietz,Hannah G Addis,Jennifer L Fox,Oleh Khalimonchuk,Laura L Lackner

Nucleic acids research 49:13108-13121 PubMed34878141

2021

FARS2 deficiency in Drosophila reveals the developmental delay and seizure manifested by aberrant mitochondrial tRNA metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlu Fan,Xiaoye Jin,Man Xu,Yongmei Xi,Weiguo Lu,Xiaohang Yang,Min-Xin Guan,Wanzhong Ge

Redox biology 46:102125 PubMed34517185

2021

Mitochondrial contact site and cristae organizing system (MICOS) machinery supports heme biosynthesis by enabling optimal performance of ferrochelatase.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan V Dietz,Mathilda M Willoughby,Robert B Piel,Teresa A Ross,Iryna Bohovych,Hannah G Addis,Jennifer L Fox,William N Lanzilotta,Harry A Dailey,James A Wohlschlegel,Amit R Reddi,Amy E Medlock,Oleh Khalimonchuk

Molecular biology of the cell 32:ar16 PubMed34432493

2021

, the conserved mitochondrial YihA GTPase family member, is required for de novo Cox1 synthesis at suboptimal temperatures in .

Applications

Unspecified application

Species

Unspecified reactive species

Yash Verma,Upasana Mehra,Dharmendra Kumar Pandey,Joy Kar,Xochitl Pérez-Martinez,Siddhartha S Jana,Kaustuv Datta

PLoS biology 19:e3001252 PubMed33983919

2021

Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition.

Applications

Unspecified application

Species

Unspecified reactive species

Pau B Esparza-Moltó,Inés Romero-Carramiñana,Cristina Núñez de Arenas,Marta P Pereira,Noelia Blanco,Beatriz Pardo,Georgina R Bates,Carla Sánchez-Castillo,Rafael Artuch,Michael P Murphy,José A Esteban,José M Cuezva
View all publications

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