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AB221766

Anti-MTERF antibody

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(1 Publication)

Rabbit Polyclonal MTERF antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTERF1 aa 1-200.

View Alternative Names

MTERF, MTERF1, Mitochondrial transcription termination factor 1, mTERF, mTERF1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MTERF antibody (AB221766)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTERF antibody (AB221766)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MTERF (green) using ab221766 at 4 μg/ml in ICC/IF.

Western blot - Anti-MTERF antibody (AB221766)
  • WB

Supplier Data

Western blot - Anti-MTERF antibody (AB221766)

All lanes:

Western blot - Anti-MTERF antibody (ab221766) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MTERF1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q99551

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MTERF (Mitochondrial Transcription Termination Factor) protein also known by alternative names like MTERF1 or h-mTERF functions mechanically by binding to mitochondrial DNA and regulating the transcription and replication processes. This protein with a mass of around 41 kDa exists predominantly in the mitochondria an organelle important for energy production in cells. MTERF plays an important role in ensuring proper mitochondrial function by terminating the transcription of mitochondrial genes.
Biological function summary

MTERF facilitates the control of gene expression within the mitochondria influencing the synthesis of essential components for the electron transport chain. It participates as part of a complex that interacts with mitochondrial DNA controlling the expression of the heavy and light strand promoters. These functions are necessary for maintaining mitochondrial DNA's integrity and proper cellular respiration.

Pathways

Mitochondrial biogenesis and homeostasis represent significant roles for MTERF particularly within the context of oxidative phosphorylation and apoptosis pathways. In oxidative phosphorylation MTERF ensures efficient energy production by regulating gene transcription. The protein interacts with other mitochondrial elements such as TFAM (Transcription Factor A Mitochondrial) and POLRMT (Mitochondrial RNA Polymerase) coordinating the replication and transcription processes.

MTERF has been connected to conditions like Mitochondrial Encephalopathy and Parkinson’s Disease. Disruptions in its function can affect mitochondrial integrity leading to neurological symptoms. MTERF is linked with proteins like COX1 and ATPase6 where its dysregulation can alter their expression contributing to disease pathogenesis. Understanding its role gives insight into developing therapeutic approaches targeting mitochondrial-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.
See full target information MTERF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110651 PubMed39262789

2024

Knockouts of , , or from cholesterol synthesis reveal pathways modulated by sterol intermediates.

Applications

Unspecified application

Species

Unspecified reactive species

Cene Skubic,Hana Trček,Petra Nassib,Tinkara Kreft,Andrew Walakira,Katka Pohar,Sara Petek,Tadeja Režen,Alojz Ihan,Damjana Rozman
View all publications
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Product promise

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