AB181836

Anti-MTERF antibody [EPR14202]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MTERF antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

MTERF, MTERF1, Mitochondrial transcription termination factor 1, mTERF, mTERF1

4 Images

Flow Cytometry (Intracellular) - Anti-MTERF antibody [EPR14202] (AB181836)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MTERF antibody [EPR14202] (AB181836)

Intracellular Flow Cytometry analysis of Jurkat cells labeling MTERF using ab181836 at a 1/50 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (AB181836)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (AB181836)

Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (AB181836)

Immunohistochemical analysis of paraffin embedded mouse muscle tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MTERF antibody [EPR14202] (AB181836)

Blocking/Dilution buffer : 5% NFDM/TBST

This antibody is not suitable to detect endogenous MTERF.

All lanes:

Western blot - Anti-MTERF antibody [EPR14202] (ab181836) at 1/1000 dilution

All lanes:

GST tagged human MTERF full length recombinant protein at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 10s

Carrier free

Anti-MTERF antibody [EPR14202] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14202

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "This antibody is not suitable to detect endogenous MTERF." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "This antibody is not suitable to detect endogenous MTERF." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MTERF (Mitochondrial Transcription Termination Factor) protein also known by alternative names like MTERF1 or h-mTERF functions mechanically by binding to mitochondrial DNA and regulating the transcription and replication processes. This protein with a mass of around 41 kDa exists predominantly in the mitochondria an organelle important for energy production in cells. MTERF plays an important role in ensuring proper mitochondrial function by terminating the transcription of mitochondrial genes.

Biological function summary

MTERF facilitates the control of gene expression within the mitochondria influencing the synthesis of essential components for the electron transport chain. It participates as part of a complex that interacts with mitochondrial DNA controlling the expression of the heavy and light strand promoters. These functions are necessary for maintaining mitochondrial DNA's integrity and proper cellular respiration.

Pathways

Mitochondrial biogenesis and homeostasis represent significant roles for MTERF particularly within the context of oxidative phosphorylation and apoptosis pathways. In oxidative phosphorylation MTERF ensures efficient energy production by regulating gene transcription. The protein interacts with other mitochondrial elements such as TFAM (Transcription Factor A Mitochondrial) and POLRMT (Mitochondrial RNA Polymerase) coordinating the replication and transcription processes.

MTERF has been connected to conditions like Mitochondrial Encephalopathy and Parkinson’s Disease. Disruptions in its function can affect mitochondrial integrity leading to neurological symptoms. MTERF is linked with proteins like COX1 and ATPase6 where its dysregulation can alter their expression contributing to disease pathogenesis. Understanding its role gives insight into developing therapeutic approaches targeting mitochondrial-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.

See full target information MTERF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta ophthalmologica 102:773-778 PubMed38553957

2024

Effect of chlorhexidine, povidone-iodine and betadine antiseptic eye drops on cultured human conjunctival goblet cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Rubin Hadad,Anne Hedengran,Alex Barnils,Goran Petrovski,Barbara Cvenkel,Tor P Utheim,Darlene A Dartt,Steffen Heegaard,Miriam Kolko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTERF antibody [EPR14202]

Flow Cytometry (Intracellular) - Anti-MTERF antibody [EPR14202] (AB181836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (AB181836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (AB181836)

Western blot - Anti-MTERF antibody [EPR14202] (AB181836)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise