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AB121910

Anti-MTERFD2 antibody

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(2 Publications)

Rabbit Polyclonal MTERFD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTERF4 aa 1-200.

View Alternative Names

MTERFD2, HSPC096, MTERF4, Mitochondrial transcription termination factor 4, mTERF domain-containing protein 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MTERFD2 antibody (AB121910)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MTERFD2 antibody (AB121910)

Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121910 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERFD2 antibody (AB121910)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERFD2 antibody (AB121910)

ab121910, at 1/60 dilution, staining MTERFD2 in paraffin embedded Human skeletal muscle tissue by Immunohistochemistry.

Western blot - Anti-MTERFD2 antibody (AB121910)
  • WB

Unknown

Western blot - Anti-MTERFD2 antibody (AB121910)

All lanes:

Western blot - Anti-MTERFD2 antibody (ab121910) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

MTERFD2 over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MTERF4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6M4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTERFD2 also known as mitochondrial transcription termination factor D2 is a human protein with a molecular mass of approximately 43 kDa. It is expressed in mitochondria specifically within the inner mitochondrial membrane. The protein plays a mechanical role in the transcription of mitochondrial DNA by influencing the termination process which helps regulate mitochondrial gene expression. MTERFD2 has a unique function compared to other MTERF family members due to its specific terminator sites.
Biological function summary

The role of MTERFD2 extends to the stabilization of mitochondrial DNA transcription processes. It is a part of the MTERF protein family which includes other transcription termination factors like MTERFD1 and MTERFD3. These proteins function together but MTERFD2 has a distinct activity in managing the balance and levels of various mitochondrial transcripts. This ensures proper mitochondrial function and energy production which are important for cell survival and metabolism.

Pathways

MTERFD2 is involved in the mitochondrial gene expression pathway and mitochondrial biogenesis. The protein assists in maintaining mitochondrial homeostasis which relates closely with other proteins like POLRMT the mitochondrial RNA polymerase. This interaction facilitates the transcriptional dynamics needed for efficient mitochondrial operation highlighting MTERFD2's necessary role in cellular energy pathways.

Research links MTERFD2 to metabolic disorders and neurodegenerative diseases. Alterations in MTERFD2 function can impair mitochondrial transcription and energy production contributing to conditions like mitochondrial myopathy. It also shows connections with proteins such as TFAM another mitochondrial transcription factor which affects similar mitochondrial functions. Understanding MTERFD2's role in these contexts is essential for developing therapeutic strategies for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of mitochondrial ribosome biogenesis and translation. Binds to mitochondrial ribosomal RNAs 16S, 12S and 7S and targets NSUN4 RNA methyltransferase to the mitochondrial large ribosomal subunit (39S).
See full target information MTERF4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:833495 PubMed35401690

2022

TMEM199-Congenital Disorder of Glycosylation With Novel Phenotype and Genotype in a Chinese Boy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fang,Kuerbanjiang Abuduxikuer,Yi-Zhen Wang,Shao-Mei Li,Lian Chen,Jian-She Wang

Molecular & cellular proteomics : MCP 19:490-500 PubMed31924693

2020

Integration of IgA and IgG Autoantigens Improves Performance of Biomarker Panels for Early Diagnosis of Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Pan,Lili Yu,Qingwei Wu,Xiaoqing Lin,Shuang Liu,Shaohui Hu,Christian Rosa,Daniel Eichinger,Ignacio Pino,Heng Zhu,Jiang Qian,Yi Huang
View all publications
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