Anti-MTF1 Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 Antibody (AB327213)

Immunohistochemical staining of human endometrium using ab327213 at 1 : 20–1 : 50 dilution, showing weak positivity in nuclear membrane in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 Antibody (AB327213)

Immunohistochemical staining of human fallopian tube using ab327213 at 1 : 20–1 : 50 dilution, showing strong cytoplasmic positivity in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 Antibody (AB327213)

Immunohistochemical staining of human pancreas using ab327213 at 1 : 20–1 : 50 dilution, showing weak cytoplasmic positivity in exocrine glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 Antibody (AB327213)

Immunohistochemical staining of human testis using ab327213 at 1 : 20–1 : 50 dilution, showing strong cytoplasmic positivity in cells in seminiferous ducts.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MTF1 Antibody (AB327213)

Immunofluorescent staining of human cell line U-251 MG using ab327213 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• ChIP

Supplier Data

ChIP - Anti-MTF1 Antibody (AB327213)

ChIP-Exo-Seq composite graph for ab327213 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-MTF1 Antibody (AB327213)

Western blot analysis in control (vector only transfected HEK293T lysate) and MTF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) using ab327213 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-MTF1 Antibody (ab327213)

Lane 2:

Control (vector only transfected HEK293T lysate)

Lane 3:

MTF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Predicted band size: 80.96 kDa

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