AB236401

Anti-MTF1 antibody [OTI2F3]

Name: Anti-MTF1 antibody [OTI2F3] (ab236401) | Abcam
Brand: Abcam Limited
SKU: AB236401
Price: 500 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal MTF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTF1 aa 1-300.

View Alternative Names

Metal regulatory transcription factor 1, MRE-binding transcription factor, Transcription factor MTF-1, MTF1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human ovary tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human lung carcinoma tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human prostate carcinoma tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human kidney tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human liver carcinoma tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human bladder carcinoma tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human prostate tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Paraffin-embedded human ovary adenocarcinoma tissue stained for MTF1 using ab236401 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MTF1 antibody [OTI2F3] (AB236401)

All lanes:

Western blot - Anti-MTF1 antibody [OTI2F3] (ab236401) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysates transfected with pCMV6-ENTRY control cDNA for 48 hrs at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY MTF1cDNA for 48 hrs at 5 µg

Predicted band size: 81 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2F3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MTF1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q14872

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.5 before commencing with IHC staining protocol.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The metallothionein transcription factor 1 also known as MTF1 is a zinc finger protein known for binding to metal-responsive elements (MREs) in the promoter region of target genes. MTF1 has a molecular mass of approximately 80 kDa. It is widely expressed in various tissues with abundant presence in organs like the liver and kidney where it plays a role in the cellular response to metal ions. This transcription factor is critical in modulating genes involved in metal ion homeostasis and detoxification.

Biological function summary

MTF1 regulates the expression of genes imperative for cellular metabolism and protection against oxidative stress. MTF1 functions as a monomer but may also interact with other transcription factors and regulators to exert its effects. Through its activity MTF1 mitigates the cellular damage induced by excess metal ions by upregulating metallothionein genes which bind and sequester metal ions. This activity assists in maintaining metal ion balance within the cell and reducing metal-induced oxidative stress.

Pathways

MTF1 plays a significant role in copper and zinc ion homeostasis pathways. It interacts with proteins such as metallothioneins (MTs) and zinc transporter proteins to regulate intracellular metal concentration. MTF1 also participates in the oxidative stress response pathway where it collaborates with other transcription factors like Nrf2 to activate the transcription of antioxidant genes. This involvement positions MTF1 as an important player in cellular defense mechanisms against oxidative damage.

Abnormal MTF1 activity has connections to certain types of cancer and neurodegenerative conditions like Alzheimer's disease. In cancer altered MTF1 expression can contribute to tumor progression by disrupting metal ion regulation which in turn affects cell proliferation and survival. It is associated with proteins like p53 which have roles in cell cycle and apoptosis. In neurodegenerative diseases impaired MTF1 function may lead to metal ion imbalance exacerbating oxidative stress and neuronal damage often in conjunction with dysregulated expression of metallothioneins and other metal-handling proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Zinc-dependent transcriptional regulator of cellular adaption to conditions of exposure to heavy metals (PubMed : 8065932). Binds to metal responsive elements (MRE) in promoters and activates the transcription of metallothionein genes like metallothionein-2/MT2A (PubMed : 8065932). Also regulates the expression of metalloproteases in response to intracellular zinc and functions as a catabolic regulator of cartilages (By similarity).

See full target information MTF1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 16:20417314251315185 PubMed39882545

2025

Therapeutic potential and mechanisms of umbilical cord mesenchymal stem cells differentiating into tendon cells and promotion of rotator cuff tendon-bone healing.

Applications

Unspecified application

Species

Unspecified reactive species

Youliang Shen,Yuelei Wang,Yidan Xu,Jie Wang,Chuqiang Yin,Zengshuai Han,Feng Shen,Ting Wang

Aging 15:14242-14262 PubMed38085674

2023

Two lncRNA signatures with cuproptosis as a novel prognostic model and clinicopathological value for endometrioid endometrial adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongrong Wu,Ruilin Lin,Liangli Hong

Nature cell biology 24:74-87 PubMed35027733

2022

The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1.

Applications

Unspecified application

Species

Unspecified reactive species

Han Han,Hiroki J Nakaoka,Line Hofmann,Jeff Jiajing Zhou,Clinton Yu,Lisha Zeng,Junyu Nan,Gayoung Seo,Rebecca Elizabeth Vargas,Bing Yang,Ruxi Qi,Lee Bardwell,Dmitry A Fishman,Ken W Y Cho,Lan Huang,Ray Luo,Rahul Warrior,Wenqi Wang

Oxidative medicine and cellular longevity 2020:3035624 PubMed32952848

2020

lncRNA OTUD6B-AS1 Exacerbates AsO-Induced Oxidative Damage in Bladder Cancer via miR-6734-5p-Mediated Functional Inhibition of IDH2.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Wang,Tianyao Yang,Yanshou Han,Zhaozhou Ren,Jiayun Zou,Jieyu Liu,Shuhua Xi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTF1 antibody [OTI2F3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

Western blot - Anti-MTF1 antibody [OTI2F3] (AB236401)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise