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Rabbit Recombinant Monoclonal MTF2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-MTF2 antibody [EPR27196-167] (AB314753), expandable thumbnail
  • Western blot - Anti-MTF2 antibody [EPR27196-167] (AB314753), expandable thumbnail
  • Western blot - Anti-MTF2 antibody [EPR27196-167] (AB314753), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MTF2 antibody [EPR27196-167] (AB314753), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (AB314753), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PIPFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity (PubMed:23142980, PubMed:23228662, PubMed:31959557). Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation (By similarity). Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells (By similarity). Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex (By similarity). In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally (By similarity). Binds to the metal-regulating-element (MRE) of MT1A gene promoter (By similarity).

Alternative names

PCL2, MTF2, Metal-response element-binding transcription factor 2, Metal regulatory transcription factor 2, Metal-response element DNA-binding protein M96, Polycomb-like protein 2, hPCl2

Recommended products

Rabbit Recombinant Monoclonal MTF2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27196-167
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human IHC and ICC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MTF2 also known as metal response element binding transcription factor 2 or M96 is a component of the Polycomb group (PcG) proteins. It's a protein involved in the regulation of gene expression via chromatin remodeling. MTF2 has a mass of approximately 110 kDa. It is expressed in various tissues with significant presence in the brain and embryonic stem cells where regulatory control of gene expression plays an important role in development.

Biological function summary

MTF2 acts mainly as a recruiter of Polycomb Repressive Complex (PRC) 2 to specific gene loci. This recruitment is essential for the methylation of histone H3 at lysine 27 (H3K27me3) a modification linked to transcriptional repression. MTF2’s role in gene silencing involves interaction with multiple other PcG proteins like EZH2 a component of PRC2 which further emphasizes its central position in maintaining the transcriptional repression needed for proper cell differentiation and development.

Pathways

MTF2 plays an important part in the regulation of the Wnt and Hedgehog signaling pathways. These pathways are significant in embryonic development and cell fate decisions. MTF2 interfaces with proteins such as SUZ12 within PRC2 which modulate repression activities by influencing chromatin structure in these pathways. Through these interactions MTF2 helps manage expression levels of downstream genes ultimately affecting cellular differentiation processes.

Associated diseases and disorders

MTF2 has been implicated in several conditions related to dysregulation of gene expression. For example aberrant function or expression of MTF2 can contribute to cancers due in part to its role in controlling cell proliferation through gene silencing. Additionally mutations or dysfunctions in MTF2 have been connected to neurological disorders because of its high expression in brain tissues. The relationship between MTF2 and proteins in the PRC2 complex such as EZH2 is of particular interest in research related to these diseases as they collectively contribute to the maintenance of gene expression patterns critical to both normal development and disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Low expression: brain (PMID:17266133). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: Lane 1-2: 48 seconds, Lane 3-4: 26 seconds.

    All lanes: Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate at 20 µg

    Lane 2: Rat liver tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa, 50 kDa, 60 kDa

  • Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Low expression: Mouse stomach (PMID:17266133).In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: Lane 1-2: 15 seconds, Lane 3: 59 seconds.

    All lanes: Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753) at 1/1000 dilution

    Lane 1: Mouse thymus tissue lysate at 20 µg

    Lane 2: Mouse stomach tissue lysate at 20 µg

    Lane 3: Human liver cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50 kDa, 60 kDa, 75 kDa

  • Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    Exposure time: Lane 1: 3 seconds, Lane 2: 10 seconds, Lane 3-4: 59 seconds.

    All lanes: Western blot - Anti-MTF2 antibody [EPR27196-167] (ab314753) at 1/1000 dilution

    Lane 1: ES-D3 (mouse embryonic pluripotent stem Cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50 kDa, 60 kDa, 75 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling MTF2 with ab314753 at 1/50 (10.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
    Confocal image showing nuclear staining in ES-D3 [D3] cells and no staining in mouse primary neuron.Low expression: mouse primary neuron.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Low expression: almost no staining on rat cerebrum. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Low expression: almost no staining mouse cerebrum. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Positive staining on mouse thymus. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Positive staining on rat thymus. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] (ab314753)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Positive staining on mouse testis. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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