Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Low expression : almost no staining on rat cerebrum. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Low expression : almost no staining mouse cerebrum. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse thymus. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat thymus. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling MTF2 with ab314753 at 1/500 (1.056 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab314753 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling MTF2 with ab314753 at 1/50 (10.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in ES-D3 [D3] cells and no staining in mouse primary neuron.Low expression : mouse primary neuron.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : Lane 1 : 3 seconds, Lane 2 : 10 seconds, Lane 3-4 : 59 seconds.

All lanes:

Western blot - Anti-MTF2 antibody [EPR27196-167] (<a href='/en-us/products/primary-antibodies/mtf2-antibody-epr27196-167-ab314753'>ab314753</a>) at 1/1000 dilution

Lane 1:

ES-D3 (mouse embryonic pluripotent stem Cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,60 kDa,75 kDa

false

• WB

Supplier Data

Western blot - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : Mouse stomach (PMID : 17266133).In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1-2 : 15 seconds, Lane 3 : 59 seconds.

All lanes:

Western blot - Anti-MTF2 antibody [EPR27196-167] (<a href='/en-us/products/primary-antibodies/mtf2-antibody-epr27196-167-ab314753'>ab314753</a>) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 20 µg

Lane 2:

Mouse stomach tissue lysate at 20 µg

Lane 3:

Human liver cancer tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,60 kDa,75 kDa

false

• WB

Supplier Data

Western blot - Anti-MTF2 antibody [EPR27196-167] - BSA and Azide free (AB314754)

This data was developed using ab314753, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : brain (PMID : 17266133). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1-2 : 48 seconds, Lane 3-4 : 26 seconds.

All lanes:

Western blot - Anti-MTF2 antibody [EPR27196-167] (<a href='/en-us/products/primary-antibodies/mtf2-antibody-epr27196-167-ab314753'>ab314753</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,50 kDa,60 kDa

false