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AB243815

Anti-MTFMT/FMT antibody

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Rabbit Polyclonal MTFMT/FMT antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MTFMT aa 50-200.

View Alternative Names

FMT, FMT1, MTFMT, MtFMT

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MTFMT/FMT antibody (AB243815)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTFMT/FMT antibody (AB243815)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling MTFMT/FMT using ab243815 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTFMT/FMT antibody (AB243815)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTFMT/FMT antibody (AB243815)

Formalin-fixed, paraffin-embedded human stomach tissue stained for MTFMT/FMT with ab243815 at a 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MTFMT aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q96DP5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTFMT also known as mitochondrial methionyl-tRNA formyltransferase or FMT is an enzyme that plays an important role in protein translation within mitochondria. It adds a formyl group to the initiator methionyl-tRNA which is essential for protein synthesis. MTFMT weighs about 43 kDa and is predominantly expressed in mitochondrial regions of human cells. This enzyme functions in concert with other mitochondrial proteins to ensure efficient initiation of protein translation.
Biological function summary

The enzyme contributes significantly to the process of mitochondrial protein biosynthesis. By formylating methionyl-tRNA MTFMT facilitates the correct start of translation which is critical for mitochondrial function. It does not seem to form part of larger protein complexes but it interacts with other translation machinery proteins to support mitochondrial function effectively. Mitochondria being powerhouses of cells rely on such proteins for the generation of energy in the form of ATP.

Pathways

Methionine formylation by MTFMT integrates into the broader process of mitochondrial translation initiation. This process plays a part in the oxidative phosphorylation pathway a central component of cellular respiration. Within this pathway MTFMT's mechanism is linked to the assembly and function of the electron transport chain. Proteins like MT-ND1 and COX5A which reside in the electron transport chain rely indirectly on MTFMT's action for proper function and assembly of mitochondrial complexes.

Mutations or deficiencies in MTFMT can disrupt mitochondrial protein synthesis leading to mitochondrial diseases. One condition associated with MTFMT malfunction is Leigh syndrome a severe neurological disorder that affects energy production. Such diseases often display overlaps in symptoms linked not just to MTFMT but also to other mitochondrial-related proteins like SURF1 and COX10. These associations underline the critical role of MTFMT in maintaining mitochondrial integrity and cellular energy balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.
See full target information MTFMT
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Product promise

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