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AB128164

Anti-MTGR1 antibody

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(2 Publications)

Rabbit Polyclonal MTGR1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CBFA2T2 aa 550 to C-terminus.

View Alternative Names

EHT, MTGR1, CBFA2T2, Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85

2 Images
Immunoprecipitation - Anti-MTGR1 antibody (AB128164)
  • IP

Unknown

Immunoprecipitation - Anti-MTGR1 antibody (AB128164)

Detection of MTGR1 by Western Blot of Immunprecipitate.
ab128164 at 1 µg/ml staining MTGR1 in 293T whole cell lysate immunoprecipitated using ab128164 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : control IgG. Predicted band size : 67 kDa.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MTGR1 antibody (ab128164)

Predicted band size: 67 kDa

false

Western blot - Anti-MTGR1 antibody (AB128164)
  • WB

Unknown

Western blot - Anti-MTGR1 antibody (AB128164)

All lanes:

Western blot - Anti-MTGR1 antibody (ab128164) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 67 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CBFA2T2 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43439

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab128164 was affinity purified using an epitope specific to MTGR1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTGR1 also known as Myeloid Translocation Gene-Related Protein 1 is a transcriptional corepressor involved in gene expression regulation. This protein has an approximate mass of 80 kDa. It is expressed widely in human tissues with higher levels in hematopoietic organs like the bone marrow. MTGR1 interacts predominantly with transcription factors to modulate various biological processes through gene silencing.
Biological function summary

MTGR1 contributes to important cellular processes by acting as a negative regulator of transcription. It forms complexes with transcription factors and histone deacetylases to exert its repressive functions. This protein is a member of the ETO family of corepressors which has several members with distinct but sometimes overlapping roles. Through its interactions MTGR1 influences the transcriptional activity of many genes impacting various cellular functions.

Pathways

MTGR1 participates in the regulation of several transcriptional networks including the Notch signaling pathway. This pathway is essential for cell differentiation proliferation and apoptosis. MTGR1 closely interacts with other coregulators such as MTG8 (ETO) and MTG16 and the AML1-ETO fusion protein providing insights into the pathways’ structural complexity. These interactions highlight its role in fine-tuning the expression of target genes involved in cellular development and maintenance.

MTGR1 plays a role in hematological malignancies such as acute myeloid leukemia (AML). It interacts with proteins like AML1 to form the AML1-ETO fusion protein which is often found in leukemia cases. MTGR1 has also implications in colorectal cancer where its altered expression affects the behavior of tumor cells. These connections underline MTGR1's importance in disease mechanisms and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed : 12559562, PubMed : 15203199). Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency (PubMed : 27281218). Involved in primordial germ cell (PCG) formation. Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent manner (By similarity). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed : 23251453). May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 (AML1-MTG8/ETO fusion protein) which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity). Required for the maintenance of the secretory cell lineage in the small intestine. Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (By similarity).
See full target information CBFA2T2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:162 PubMed32133296

2020

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Engqvist,Toshima Z Parris,Anikó Kovács,Elisabeth Werner Rönnerman,Karin Sundfeldt,Per Karlsson,Khalil Helou

Cancer cell international 17:103 PubMed29162985

2017

CBFA2T2 is associated with a cancer stem cell state in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Du-Chu Chen,You-De Liang,Liang Peng,Yi-Ze Wang,Chun-Zhi Ai,Xin-Xing Zhu,Ya-Wei Yan,Yasmeen Saeed,Bin Yu,Jingying Huang,Yuxin Gao,Jiaqi Liu,Yi-Zhou Jiang,Min Liu,Demeng Chen
View all publications
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