Anti-MTHFD1 antibody

• WB

Unknown

Western blot - Anti-MTHFD1 antibody (AB103698)

All lanes:

Western blot - Anti-MTHFD1 antibody (ab103698) at 5 µg/mL

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 102 kDa

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• WB

Supplier Data

Western blot - Anti-MTHFD1 antibody (AB103698)

All lanes:

Western blot - Anti-MTHFD1 antibody (ab103698) at 5 µg/mL

Lane 1:

MTHFD1 transfected 293T cell lysate

Lane 2:

Non-transfected 293T cell lysate

Predicted band size: 102 kDa

false

• WB

Unknown

Western blot - Anti-MTHFD1 antibody (AB103698)

All lanes:

Western blot - Anti-MTHFD1 antibody (ab103698) at 5 µg/mL

All lanes:

Human liver tissue lysate at 50 µg

Predicted band size: 102 kDa

false

• WB

CiteAb

Western blot - Anti-MTHFD1 antibody (AB103698)

MTHFD1 western blot using anti-MTHFD1 antibody ab103698. Publication image and figure legend from Kühl, I., Miranda, M., et al., 2017, Elife, PubMed 29132502.

ab103698 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab103698 please see the product overview.

Up-regulation of the enzymes of the mitochondrial 1C pathway happens before deficient OXPHOS is detectable in mouse heart.(A) Scheme of 1C pathway. Colored boxes : protein levels; red : increased, grey : not detected or not quantified. (B) Heatmaps showing the fold-change in transcript (left) and protein (right) levels in alphabetical order of L/L, cre and L/L mouse hearts of the 1C pathway; p<0.0001 in≥1 knockout strain. (C) Immunoblot of enzymes of the 1C pathway in total protein extracts from L/L, cre and L/L; Loading : tubulin. (D) Quantification of 1C donor metabolite levels in L/L, cre and L/L. Graphs represent mean ± SEM (*p<0.05, **p<0.01, ***p<0.001). (E) Time point analysis of protein levels of enzymes of the 1C pathway (top), and LRPPRC and VDAC (bottom) in Lrpprc knockout hearts compared to controls. Yellow line : average value of nuclear and mitochondrial encoded OXPHOS complex IV subunits. Adjusted p<0.05, except for VDAC. LRPPRC and VDAC protein levels at the different time points were verified by immunoblot presented in Figure 6—figure supplement 1.10.7554/eLife.30952.025Figure 6—source data 1.Determination of 1C pathway donor metabolites.Determination of 1C pathway donor metabolites.Steady-state LRPPRC protein levels at different time points in mitochondrial extracts from Lrpprc L/L, cre and L/L hearts; Loading : VDAC1.

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