AB116615

Anti-MTHFD1L antibody

Name: Anti-MTHFD1L antibody (ab116615) | Abcam
Brand: Abcam Limited
SKU: AB116615
Price: 520 USD
Availability: InStock

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(1 Publication)

Goat Polyclonal MTHFD1L antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MTHFD1L aa 550-600.

View Alternative Names

FTHFSDC1, MTHFD1L, Formyltetrahydrofolate synthetase

2 Images

Supplier Data

Western blot - Anti-MTHFD1L antibody (AB116615)

Primary incubation for 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-MTHFD1L antibody (ab116615) at 0.1 µg/mL

Lane 1:

NIH3T3 cell lysate in RIPA buffer at 35 µg

Lane 2:

Mouse brain tissue lysate in RIPA buffer at 35 µg

Lane 3:

Rat brain tissue lysate in RIPA buffer at 35 µg

Predicted band size: 106 kDa,44 kDa

Observed band size: 110 kDa

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Unknown

Western blot - Anti-MTHFD1L antibody (AB116615)

Primary incubation for 1 hour.

All lanes:

Western blot - Anti-MTHFD1L antibody (ab116615) at 0.1 µg/mL

All lanes:

Human cerebellum tissue lysate in RIPA buffer at 35 µg

Predicted band size: 106 kDa

Observed band size: 110 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MTHFD1L aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q6UB35

Specificity

Based on homology with the immunogen sequence, we believe this antibody will also react with MTHFD1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab116615 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTHFD1L also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like functions as an enzyme participating in folate metabolism. With an approximate mass of 105 kDa MTHFD1L is active in the mitochondria. This enzyme facilitates the interconversion of tetrahydrofolate derivatives supporting the folate-mediated one-carbon metabolic pathway. MTHFD1L shows expression in various tissues with a noticeable presence in liver and embryonic tissues where it has roles in embryogenesis and tissue-specific functions.

Biological function summary

MTHFD1L catalyzes reactions converting methylene-THF to methenyl-THF and then to formyl-THF utilizing NADP+ as a cofactor. It does not function as a standalone component; instead it may operate as part of a larger complex engaged in one-carbon metabolism. Its activity contributes to the synthesis of important biomolecules such as nucleotides and amino acids emphasizing its role in DNA synthesis and repair.

Pathways

The folate-mediated one-carbon metabolism uses MTHFD1L to manage one-carbon units. This enzyme's function fits into the folate pathway important for nucleotide biosynthesis. It connects to other proteins such as MTHFD1 and SHMT1 which also partake in folate metabolism supporting interconnected biochemical pathways in various cellular processes.

Defects or alterations in MTHFD1L are linked to neural tube defects and some cancers. The enzyme's involvement in embryonic development and DNA repair suggests that mutations or dysregulation may contribute to these conditions. MTHFD1 and SHMT1 related proteins have similar associations highlighting their collective impact on disease when there is impaired folate metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism complementing thus the enzymatic activities of MTHFD2.

See full target information MTHFD1L

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 42: PubMed35502767

2022

Activated brown adipose tissue releases exosomes containing mitochondrial methylene tetrahydrofolate dehydrogenase (NADP dependent) 1-like protein (MTHFD1L).

Applications

Unspecified application

Species

Unspecified reactive species

Melvin Khee-Shing Leow,Anantharaj Rengaraj,Kothandaraman Narasimhan,Sanjay K Verma,Jadegoud Yaligar,Giang Le Thi Thu,Lijuan Sun,Hui Jen Goh,Priya Govindharajulu,Suresh Anand Sadananthan,Navin Michael,Wei Meng,Xavier Gallart-Palau,Lei Sun,Neerja Karnani,Newman Siu Kwan Sze,S Sendhil Velan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTHFD1L antibody

Western blot - Anti-MTHFD1L antibody (AB116615)

Western blot - Anti-MTHFD1L antibody (AB116615)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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