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AB229708

Anti-MTHFD1L antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal MTHFD1L antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFD1L aa 750 to C-terminus.

View Alternative Names

FTHFSDC1, MTHFD1L, Formyltetrahydrofolate synthetase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD1L antibody - C-terminal (AB229708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD1L antibody - C-terminal (AB229708)

Paraffin-embedded human kidney tissue stained for MTHFD1L using ab229708 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD1L antibody - C-terminal (AB229708)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD1L antibody - C-terminal (AB229708)

Paraffin-embedded human colon cancer tissue stained for MTHFD1L using ab229708 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MTHFD1L antibody - C-terminal (AB229708)
  • WB

Supplier Data

Western blot - Anti-MTHFD1L antibody - C-terminal (AB229708)

All lanes:

Western blot - Anti-MTHFD1L antibody - C-terminal (ab229708) at 1/1000 dilution

Lane 1:

Colo3320 whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 106 kDa

Observed band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MTHFD1L aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6UB35

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTHFD1L also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like functions as an enzyme participating in folate metabolism. With an approximate mass of 105 kDa MTHFD1L is active in the mitochondria. This enzyme facilitates the interconversion of tetrahydrofolate derivatives supporting the folate-mediated one-carbon metabolic pathway. MTHFD1L shows expression in various tissues with a noticeable presence in liver and embryonic tissues where it has roles in embryogenesis and tissue-specific functions.
Biological function summary

MTHFD1L catalyzes reactions converting methylene-THF to methenyl-THF and then to formyl-THF utilizing NADP+ as a cofactor. It does not function as a standalone component; instead it may operate as part of a larger complex engaged in one-carbon metabolism. Its activity contributes to the synthesis of important biomolecules such as nucleotides and amino acids emphasizing its role in DNA synthesis and repair.

Pathways

The folate-mediated one-carbon metabolism uses MTHFD1L to manage one-carbon units. This enzyme's function fits into the folate pathway important for nucleotide biosynthesis. It connects to other proteins such as MTHFD1 and SHMT1 which also partake in folate metabolism supporting interconnected biochemical pathways in various cellular processes.

Defects or alterations in MTHFD1L are linked to neural tube defects and some cancers. The enzyme's involvement in embryonic development and DNA repair suggests that mutations or dysregulation may contribute to these conditions. MTHFD1 and SHMT1 related proteins have similar associations highlighting their collective impact on disease when there is impaired folate metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism complementing thus the enzymatic activities of MTHFD2.
See full target information MTHFD1L
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 51:990-998 PubMed31133746

2019

MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Sdelci,André F Rendeiro,Philipp Rathert,Wanhui You,Jung-Ming G Lin,Anna Ringler,Gerald Hofstätter,Herwig P Moll,Bettina Gürtl,Matthias Farlik,Sandra Schick,Freya Klepsch,Matthew Oldach,Pisanu Buphamalai,Fiorella Schischlik,Peter Májek,Katja Parapatics,Christian Schmidl,Michael Schuster,Thomas Penz,Dennis L Buckley,Otto Hudecz,Richard Imre,Shuang-Yan Wang,Hans Michael Maric,Robert Kralovics,Keiryn L Bennett,Andre C Müller,Karl Mechtler,Jörg Menche,James E Bradner,Georg E Winter,Kristaps Klavins,Emilio Casanova,Christoph Bock,Johannes Zuber,Stefan Kubicek

American journal of cancer research 9:250-269 PubMed30906627

2019

MicroRNA-940 inhibits glioma progression by blocking mitochondrial folate metabolism through targeting of MTHFD2.

Applications

Unspecified application

Species

Unspecified reactive species

Tuoye Xu,Kaixin Zhang,Jing Shi,Baosheng Huang,Xi Wang,Kai Qian,Tao Ma,Tengda Qian,Zewu Song,Lixin Li
View all publications
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