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Rabbit Polyclonal MTHFD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus.


Images

Western blot - Anti-MTHFD2 antibody (AB151447), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (AB151447), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13995

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.

Alternative names

Recommended products

Rabbit Polyclonal MTHFD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13995
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.

Biological function summary

Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.

Pathways

MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.

Associated diseases and disorders

The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.

Product promise

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3 product images

  • Western blot - Anti-MTHFD2 antibody (ab151447), expandable thumbnail

    Western blot - Anti-MTHFD2 antibody (ab151447)

    10% SDS PAGE

    All lanes: Western blot - Anti-MTHFD2 antibody (ab151447) at 1/1000 dilution

    Lane 1: 293T cell lysate at 30 µg

    Lane 2: A431 cell lysate at 30 µg

    Lane 3: H1299 cell lysate at 30 µg

    Lane 4: HeLa cell lysate at 30 µg

    Lane 5: HepG2 cell lysate at 30 µg

    Lane 6: Molt4 cell lysate at 30 µg

    Lane 7: Raji cell lysate at 30 µg

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (ab151447), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (ab151447)

    Immunohistochemical analysis of paraffin-embedded Human SAS Xenograft tissue, labeling MTHFD2 with ab151447 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (ab151447), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (ab151447)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa labeling MTHFD2 with ab151447 at 1/200 dilution. The image in the lowe panel was merged with DNA probe.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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