Rabbit Polyclonal MTHFD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted | Predicted |
Xenopus tropicalis | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Select an associated product type
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
NMDMC, MTHFD2
Rabbit Polyclonal MTHFD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.
Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.
MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.
The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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10% SDS PAGE
All lanes: Western blot - Anti-MTHFD2 antibody (ab151447) at 1/1000 dilution
Lane 1: 293T cell lysate at 30 µg
Lane 2: A431 cell lysate at 30 µg
Lane 3: H1299 cell lysate at 30 µg
Lane 4: HeLa cell lysate at 30 µg
Lane 5: HepG2 cell lysate at 30 µg
Lane 6: Molt4 cell lysate at 30 µg
Lane 7: Raji cell lysate at 30 µg
Predicted band size: 38 kDa
Immunohistochemical analysis of paraffin-embedded Human SAS Xenograft tissue, labeling MTHFD2 with ab151447 at 1/500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa labeling MTHFD2 with ab151447 at 1/200 dilution. The image in the lowe panel was merged with DNA probe.
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