AB151447

Anti-MTHFD2 antibody

Name: Anti-MTHFD2 antibody (ab151447) | Abcam
Brand: Abcam Limited
SKU: AB151447
Rating: 4 (3 reviews)

(3 Reviews)

(22 Publications)

Rabbit Polyclonal MTHFD2 antibody. Suitable for IHC-P, WB, ICC/IF, IP and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus.

View Alternative Names

NMDMC, MTHFD2

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (AB151447)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (AB151447)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling MTHFD2 protein using ab151447 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

Immunohistochemical analysis of paraffin-embedded human mcf7 xenograft tissue stained for MTHFD2 protein with ab151447 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

Immunohistochemical analysis of paraffin-embedded Human SAS Xenograft tissue, labeling MTHFD2 with ab151447 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for MTHFD2 protein with ab151447 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

Supplier Data

Immunoprecipitation - Anti-MTHFD2 antibody (AB151447)

Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 µg of ab151447. Western blot analysis was performed using ab151447.

All lanes:

Immunoprecipitation - Anti-MTHFD2 antibody (ab151447) at 5 µg

Lane 1:

MTHFD2 protein from 293T whole cell extract with control IgG

Lane 2:

MTHFD2 protein from 293T whole cell extract

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-MTHFD2 antibody (AB151447)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-MTHFD2 antibody (ab151447) at 1/3000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

shRNA transfected 293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-MTHFD2 antibody (AB151447)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-MTHFD2 antibody (ab151447) at 1/1000 dilution

Lane 1:

293T whole cell extract at 30 µg

Lane 2:

A431 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human MTHFD2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.

Biological function summary

Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.

Pathways

MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.

The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.

See full target information MTHFD2

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6613 PubMed40738970

2025

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects.

Applications

Unspecified application

Species

Unspecified reactive species

Ritobrata Ghose,Fabio Pezzano,Rémi Badia,Savvas Kourtis,Ilir Sheraj,Shubhamay Das,Antoni Gañez Zapater,Upamanyu Ghose,Sara Musa-Afaneh,Lorena Espinar,Albert Coll-Manzano,Katja Parapatics,Saška Ivanova,Paula Sànchez-Fernàndez-de-Landa,Dragana Radivojevikj,Valeria Venturini,Stefan Wieser,Antonio Zorzano,André C Müller,Verena Ruprecht,Sara Sdelci

Clinical and translational medicine 15:e70404 PubMed40681476

2025

PDK4 and nutrient responses explain muscle specific manifestation in mitochondrial disease.

Applications

Unspecified application

Species

Unspecified reactive species

Swagat Pradhan,Takayuki Mito,Nahid A Khan,Sofiia Olander,Aleksandra Zhaivoron,Thomas G McWilliams,Anu Suomalainen

Cancer medicine 13:e70349 PubMed39540204

2024

A Novel Gene Expression Scoring System Predicts Recurrence in Non-Muscle-Invasive Bladder Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Emina Kayama,Motohide Uemura,Akifumi Onagi,Satoru Meguro,Soichiro Ogawa,Kei Yaginuma,Kanako Matsuoka,Seiji Hoshi,Tomoyuki Koguchi,Junya Hata,Yuichi Sato,Hidenori Akaihata,Reiko Honma,Shinya Watanabe,Yoshiyuki Kojima

Nature communications 15:9529 PubMed39532843

2024

Nuclear localization of MTHFD2 is required for correct mitosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Pardo-Lorente,Anestis Gkanogiannis,Luca Cozzuto,Antoni Gañez Zapater,Lorena Espinar,Ritobrata Ghose,Jacqueline Severino,Laura García-López,Rabia Gül Aydin,Laura Martin,Maria Victoria Neguembor,Evangelia Darai,Maria Pia Cosma,Laura Batlle-Morera,Julia Ponomarenko,Sara Sdelci

Cell death & disease 15:774 PubMed39455562

2024

One-carbon-mediated purine synthesis underlies temozolomide resistance in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kimia Ghannad-Zadeh,Alyona Ivanova,Megan Wu,Taylor M Wilson,Alyssa Lau,Robert Flick,David G Munoz,Sunit Das

Biochimica et biophysica acta. Molecular basis of disease 1870:167131 PubMed38521420

2024

A stagewise response to mitochondrial dysfunction in mitochondrial DNA maintenance disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Vincent,Chun Chen,Tiago Bernardino Gomes,Valeria Di Leo,Tuomas Laalo,Kamil Pabis,Rodrick Capaldi,Michael F Marusich,David McDonald,Andrew Filby,Andrew Fuller,Diana Lehmann Urban,Stephan Zierz,Marcus Deschauer,Doug Turnbull,Amy K Reeve,Conor Lawless

Nature communications 15:1163 PubMed38331894

2024

Unraveling the role of the mitochondrial one-carbon pathway in undifferentiated thyroid cancer by multi-omics analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Eun Lee,Seongyeol Park,Shinae Yi,Na Rae Choi,Mi Ae Lim,Jae Won Chang,Ho-Ryun Won,Je Ryong Kim,Hye Mi Ko,Eun-Jae Chung,Young Joo Park,Sun Wook Cho,Hyeong Won Yu,June Young Choi,Min-Kyung Yeo,Boram Yi,Kijong Yi,Joonoh Lim,Jun-Young Koh,Min Jeong Lee,Jun Young Heo,Sang Jun Yoon,Sung Won Kwon,Jong-Lyul Park,In Sun Chu,Jin Man Kim,Seon-Young Kim,Yujuan Shan,Lihua Liu,Sung-A Hong,Dong Wook Choi,Junyoung O Park,Young Seok Ju,Minho Shong,Seon-Kyu Kim,Bon Seok Koo,Yea Eun Kang

Biology direct 18:73 PubMed37946250

2023

p63 orchestrates serine and one carbon metabolism enzymes expression in head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Cappello,Giulia Tosetti,Artem Smirnov,Carlo Ganini,Xue Yang,Yufang Shi,Ying Wang,Gerry Melino,Francesca Bernassola,Eleonora Candi

Frontiers in immunology 14:1209490 PubMed37457690

2023

The actions of methotrexate on endothelial cells are dependent on the shear stress-induced regulation of one carbon metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marie B Lang,Kit-Yi Leung,Nicholas D E Greene,Kerri M Malone,Gaye Saginc,Anna M Randi,Allan Kiprianos,Robert T Maughan,Charis Pericleous,Justin C Mason

Cell death discovery 9:102 PubMed36964165

2023

Serine and one-carbon metabolism sustain non-melanoma skin cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Cappello,Alessandro Zuccotti,Mara Mancini,Giulia Tosetti,Luca Fania,Francesco Ricci,Gerry Melino,Eleonora Candi

View all publications

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Product promise

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Anti-MTHFD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (AB151447)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (AB151447)

Immunoprecipitation - Anti-MTHFD2 antibody (AB151447)

Western blot - Anti-MTHFD2 antibody (AB151447)

Western blot - Anti-MTHFD2 antibody (AB151447)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise