Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)

Rabbit Recombinant Monoclonal MTHFD2 antibody. Carrier free. Suitable for IHC-P, IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (AB307429), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt (Intra)	ICC/IF	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Expected	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information MTHFD2

Function

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.

Alternative names

NMDMC, MTHFD2

Recommended products

Rabbit Recombinant Monoclonal MTHFD2 antibody. Carrier free. Suitable for IHC-P, IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR26938-20
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.

Biological function summary

Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.

Pathways

MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.

Associated diseases and disorders

The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
MTHFD2 was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast) whole cell lysate 10 ug with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: MEF (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2: ABAB307428 IP in MEF whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MTHFD2 antibody [EPR26938-20] ab307428 in MEF whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
All lanes: Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] (Anti-MTHFD2 antibody [EPR26938-20] ab307428) at 1/1000 dilution
Lane 1: MEF (mouse embryonic fibroblast) whole cell lysate 10 μg
Lane 2: MEF whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 35 kDa
Exposure time: 10s
Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
MTHFD2 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB307428 IP in HEK-293 whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MTHFD2 antibody [EPR26938-20] ab307428 in HEK-293 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
All lanes: Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] (Anti-MTHFD2 antibody [EPR26938-20] ab307428) at 1/1000 dilution
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2: HEK-293 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 35 kDa
Exposure time: 1s
Western blot - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using 307428, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: In Western blot, Anti-MTHFD2 antibody [EPR26938-20] ab307428 was shown to bind specifically to MTHFD2. A band was observed at 35 kDa in wild-type HEK-293 cell lysates whereas no signal observed at this size in MTHFD2 knockout cell line Human MTHFD2 knockout HEK-293T cell line ab266383 (knockout cell lysate Human MTHFD2 knockout HEK-293T cell lysate ab258528).
15 seconds
Exposure time:
All lanes: Western blot - Anti-MTHFD2 antibody [EPR26938-20] (Anti-MTHFD2 antibody [EPR26938-20] ab307428) at 1/1000 dilution
Lane 1: Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 2: MTHFD2 knockout HEK-293 whole cell lysate 20 μg
Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 4: MEF (mouse embryonic fibroblast) whole cell lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35 kDa
Exposure time: 15s
Western blot - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using 307428, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The Lanes 2-6 were developed using a high sensitivity ECL substrate.

Exposure time:
All lanes: Western blot - Anti-MTHFD2 antibody [EPR26938-20] (Anti-MTHFD2 antibody [EPR26938-20] ab307428) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate 20 μg
Lane 2: Human colon tissue lysate 20 μg
Lane 3: Human colon cancer tissue lysate 20 μg
Lane 4: Human testis tissue lysate 20 μg
Lane 5: Human ovary tissue lysate 20 μg
Lane 6: Mouse breast cancer tissue lysate 20 μg
Lane 7: Mouse pancreas tissue lysate 20 μg
Lane 8: Mouse kidney tissue lysate 20 μg
Lane 9: Rat kidney tissue lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 35 kDa
Exposure time: 15s
Western blot - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using 307428, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
Lane 1: 15 seconds, Lanes 2-6 and 8-9: 180 seconds, Lane 7: 48 seconds.
Exposure time:
All lanes: Western blot - Anti-MTHFD2 antibody [EPR26938-20] (Anti-MTHFD2 antibody [EPR26938-20] ab307428) at 1/1000 dilution
Lane 1: 786-O (Human kidney renal cell adenocarcinoma) whole cell lysate 20 μg
Lane 2: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35 kDa
Exposure time: 48s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat spleen.The section was incubated with Anti-MTHFD2 antibody [EPR26938-20] ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse spleen.The section was incubated with Anti-MTHFD2 antibody [EPR26938-20] ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human tonsil (PMID: 24451681).The section was incubated with Anti-MTHFD2 antibody [EPR26938-20] ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human breast cancer (PMID: 26461067).The section was incubated with Anti-MTHFD2 antibody [EPR26938-20] ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 tissue labeling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on (A) Wild-type HEK293T (human embryonic kidney epithelial cell) cell pellet, no staining on (B) MTHFD2 knockout HEK293T (Human MTHFD2 knockout HEK-293T cell line ab266383) cell pellet.The section was incubated with Anti-MTHFD2 antibody [EPR26938-20] ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized MTHFD2 KO HEK293T (MTHFD2 knockout human embryonic kidney epithelial cell), Human MTHFD2 knockout HEK-293T cell line ab266383 cells lABelling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/1000 (0.504 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in wildtype HEK293T cells and no staining in MTHFD2 knockout HEK293T cells.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab197491 Anti-COX IV Mouse monoclonal antibody (Alexa Fluor? 647) - Mitochondrial Marker was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized MEF (mouse embryo fibroblast) cells lABelling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/1000 (0.504 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in MEF cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Wild-type 293T (human embryonic kidney epithelial cell, Right) / MTHFD2 knockout 293T (Left) cells lABelling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/5000 dilution (0.01ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Positive staining on 293T cells (ab255449), while no staining on MTHFD2 knockout 293T cells (Human MTHFD2 knockout HEK-293T cell line ab266383).
Flow Cytometry (Intracellular) - Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free (ab307429)
This data was developed using Anti-MTHFD2 antibody [EPR26938-20] ab307428, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized MEF (mouse embryonic fibroblast (immortalized)) cells lABelling MTHFD2 with Anti-MTHFD2 antibody [EPR26938-20] ab307428 at 1/500 dilution (0.1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.