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AB307905

Anti-MTHFD2 antibody [EPR26938-38] - BSA and Azide free (Detector)

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Knockout Tested Rabbit Recombinant Monoclonal MTHFD2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

NMDMC, MTHFD2

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Sandwich ELISA - Anti-MTHFD2 antibody [EPR26938-38] - BSA and Azide free (Detector) (AB307905)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MTHFD2 antibody [EPR26938-38] - BSA and Azide free (Detector) (AB307905)

Sandwich ELISA of ab307903 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Sandwich ELISA - Anti-MTHFD2 antibody [EPR26938-38] - BSA and Azide free (Detector) (AB307905)
  • sELISA

Lab

Sandwich ELISA - Anti-MTHFD2 antibody [EPR26938-38] - BSA and Azide free (Detector) (AB307905)

Interpolated concentrations of native MTHFD2 in human control wild type HEK293T (human embryonic kidney epithelial cell) and MTHFD2 knockout HEK293T cell based on 2.5 µg/mL extract loads. The concentrations of MTHFD2 were measured in duplicate and interpolated from the MTHFD2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MTHFD2 concentration was determined to be 2470.7 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in MTHFD2 knockout HEK293T extract (Human MTHFD2 knockout HEK293T cell line ab266383).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26938-38

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTHFD2 also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 is an enzyme with a mass of approximately 36 kDa. It catalyzes the conversion of methylenetetrahydrofolate to methenyltetrahydrofolate using NADP+ as a cofactor. MTHFD2 is a mitochondrial protein and is highly expressed in embryonic tissues and various cancers while showing low expression in normal adult tissues. This differential expression makes it an interesting target for cancer research.
Biological function summary

Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.

Pathways

MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.

The upregulation of MTHFD2 strongly associates with various cancers including breast and colorectal cancers. The involvement of MTHFD2 in these cancers indicates its potential as a biomarker and therapeutic target. Through cancer-related pathways MTHFD2 shows a connection to enzymes such as ALDH1L2 (aldehyde dehydrogenase 1 family member L2) which also participates in folate metabolism and influences cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
See full target information MTHFD2
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Product promise

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For full details, please see our Terms & Conditions

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