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AB236737

Anti-MTHFR antibody

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(1 Publication)

Rabbit Polyclonal MTHFR antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFR aa 1 to C-terminus.

View Alternative Names

Methylenetetrahydrofolate reductase (NADPH), MTHFR

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MTHFR antibody (AB236737)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTHFR antibody (AB236737)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for MTHFR (green) using ab236737 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody (AB236737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody (AB236737)

Paraffin-embedded human prostate cancer tissue stained for MTHFR using ab236737 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MTHFR antibody (AB236737)
  • WB

Supplier Data

Western blot - Anti-MTHFR antibody (AB236737)

All lanes:

Western blot - Anti-MTHFR antibody (ab236737) at 1/500 dilution

All lanes:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MTHFR aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P42898

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'MTHFR' protein also known as methylenetetrahydrofolate reductase plays an important role in folate metabolism by catalyzing the reduction of 510-methylenetetrahydrofolate to 5-methyltetrahydrofolate. This enzyme has a mass of approximately 70 kDa and is expressed in various tissues including liver kidney and brain. It exists primarily in the cytoplasm where it performs its enzymatic functions critical for cellular metabolism.
Biological function summary

Methylenetetrahydrofolate reductase is essential in the process of transferring one-carbon units especially in the synthesis of the amino acid methionine and the methylation of DNA. This enzyme does not form part of a larger protein complex but operates independently in the folate cycle. Its activity directly influences homocysteine levels within the cell with alterations in its function affecting many metabolic processes.

Pathways

Methylenetetrahydrofolate reductase is an important component of the folate and methionine cycles significant in the remethylation of homocysteine to methionine. These pathways intersect with transmethylation and nucleotide synthesis essential for cell functions and replication. It interacts with proteins like methionine synthase and cystathionine beta-synthase which further demonstrate the enzyme's importance in maintaining cellular homeostasis.

Methylenetetrahydrofolate reductase deficiency or mutations often lead to hyperhomocysteinemia and have been associated with cardiovascular diseases and neurological disorders. Mutations in this enzyme can alter its efficiency leading to the accumulation of homocysteine. This has connections with proteins such as methionine synthase where impaired functioning due to MTHFR mutation can exacerbate the disruption of homocysteine metabolism contributing to disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine (PubMed : 29891918). Represents a key regulatory connection between the folate and methionine cycles (Probable).
See full target information MTHFR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112974 PubMed40687831

2025

Mutation T9I in Envelope confers autophagy resistance to SARS-CoV-2 Omicron.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Klute,Rayhane Nchioua,Arne Cordsmeier,Jyoti Vishwakarma,Lennart Koepke,Hala Alshammary,Christoph Jung,Maximilian Hirschenberger,Helene Hoenigsperger,Jana-Romana Fischer,Rinu Sivarajan,Fabian Zech,Steffen Stenger,Ruth Serra-Moreno,Ana Silvia Gonzalez-Reiche,Emilia Mia Sordillo,Harm van Bakel,Viviana Simon,Frank Kirchhoff,Timo Jacob,Dorota Kmiec,Andreas Pichlmair,Armin Ensser,Konstantin Maria Johannes Sparrer
View all publications
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Product promise

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