AB230321

Anti-MTIF3 antibody

Name: Anti-MTIF3 antibody (ab230321) | Abcam
Brand: Abcam Limited
SKU: AB230321

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(1 Publication)

Rabbit Polyclonal MTIF3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MTIF3.

View Alternative Names

DC38, MTIF3, IF-3(Mt), IF-3Mt, IF3(mt), IF3mt

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTIF3 antibody (AB230321)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTIF3 antibody (AB230321)

Paraffin-embedded human lung cancer tissue stained for MTIF3 using ab230321 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTIF3 antibody (AB230321)

Paraffin-embedded human prostate cancer tissue stained for MTIF3 using ab230321 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MTIF3 antibody (AB230321)

All lanes:

Western blot - Anti-MTIF3 antibody (ab230321) at 1/1000 dilution

Lane 1:

Mouse small intestine lysate

Lane 2:

Rat heart lysate

Lane 3:

Mouse liver lysate

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MTIF3.

Q9H2K0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTIF3 also known as mitochondrial translational initiation factor 3 (IF-3mt) weighs approximately 29 kDa. This protein facilitates the initiation of translation within mitochondria by ensuring the correct assembly of ribosomes. MTIF3 expression occurs in tissues with high energy demands such as heart and skeletal muscles due to its role in mitochondrial function. Notably the protein aids in disassociation of the ribosomal subunits enabling the correct positioning of mRNA on the ribosome.

Biological function summary

Mitochondrial translation regulation relies on MTIF3's interaction with other translation factors. MTIF3 as part of the mitochondrial translation initiation complex interacts with mitochondrial ribosomes to prepare them for protein synthesis. This protein regulates the precision of translation initiation ensuring that the translation machinery only begins under appropriate conditions. MTIF3 binds to the small ribosomal subunit playing a guiding role at the beginning of mitochondrial translation.

Pathways

MTIF3 integrates into processes involving mitochondrial protein synthesis and energy production. MTIF3's function significantly affects key pathways like oxidative phosphorylation as it ensures the efficient production of mitochondrial proteins needed for this process. Its association with other factors such as MTIF2 another initiation factor further characterizes its role in this pathway. Together these proteins are critical for the production of proteins that compose the electron transport chain necessary for ATP generation.

MTIF3 has implications for conditions linked to mitochondrial dysfunction. Researchers have identified its role in mitochondrial cardiomyopathy where defects in MTIF3 may lead to impaired cardiac energy metabolism. Potential connections also exist between MTIF3 and certain mitochondrial encephalomyopathies where its impact on mitochondrial translation affects neurological function. Disruptions in MTIF3 levels may alter the expression of other mitochondrial proteins exacerbating such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

IF-3 binds to the 28S ribosomal subunit and shifts the equilibrium between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.

See full target information MTIF3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC genomics 20:363 PubMed31072381

2019

Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops.

Applications

Unspecified application

Species

Unspecified reactive species

Junlin Song,Chunde Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTIF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTIF3 antibody (AB230321)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTIF3 antibody (AB230321)

Western blot - Anti-MTIF3 antibody (AB230321)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise