Anti-mTOR antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody - C-terminal (AB137341)

Immunofluorescence analysis of ice-cold MeOH fixed for 5 mins MCF-7 cells labelling mTOR protein at cytoplasm using ab137341 at a 1/2000 dilution (Green). The nuclear counterstain is Hoechst 33342 (Blue). Scale bar= 10 µm.

• ChIP

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ChIP - Anti-mTOR antibody - C-terminal (AB137341)

Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or ab137341. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .

• IP

Supplier Data

Immunoprecipitation - Anti-mTOR antibody - C-terminal (AB137341)

Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of ab137341. Western blot analysis was performed using ab137341.

All lanes:

Immunoprecipitation - Anti-mTOR antibody - C-terminal (ab137341)

Lane 1:

293T Whole cell extract (Input)

Lane 2:

Control IgG

Lane 3:

293T Whole cell extract (+)

Secondary

All lanes:

Anti-Rabbit IgG

false

• IP

Supplier Data

Immunoprecipitation - Anti-mTOR antibody - C-terminal (AB137341)

mTOR antibody immunoprecipitates mTOR protein using 293T whole cell lysate/extract. In western blot, the immunoprecipitated mTOR protein was detected by mTOR antibody diluted at 1 : 1000.

All lanes:

Immunoprecipitation - Anti-mTOR antibody - C-terminal (ab137341) at 1/1000 dilution

Lane 1:

Control with 3 μg of pre-immune rabbit IgG

Lane 2:

293T whole cell lysate/extract

Secondary

All lanes:

Anti-rabbit IgG (HRP) conjugate

Predicted band size: 289 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR antibody - C-terminal (AB137341)

Paraffin-embedded mouse testis tissue stained for mTOR protein at cytoplasm in mouse testis with ab137341 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

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Western blot - Anti-mTOR antibody - C-terminal (AB137341)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program

All lanes:

Western blot - Anti-mTOR antibody - C-terminal (ab137341) at 1/500 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa Whole cell extract at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 289 kDa

false

• WB

Supplier Data

Western blot - Anti-mTOR antibody - C-terminal (AB137341)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-mTOR antibody - C-terminal (ab137341) at 1/500 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 289 kDa

false

• WB

Supplier Data

Western blot - Anti-mTOR antibody - C-terminal (AB137341)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-mTOR antibody - C-terminal (ab137341) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Predicted band size: 289 kDa

false

• WB

Supplier Data

Western blot - Anti-mTOR antibody - C-terminal (AB137341)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-mTOR antibody - C-terminal (ab137341) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 289 kDa

false

• WB

Supplier Data

Western blot - Anti-mTOR antibody - C-terminal (AB137341)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-mTOR antibody - C-terminal (ab137341) at 1/1000 dilution

All lanes:

C6 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 289 kDa

false

• WB

Supplier Data

Western blot - Anti-mTOR antibody - C-terminal (AB137341)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-mTOR antibody - C-terminal (ab137341) at 1/500 dilution

All lanes:

Mouse brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 289 kDa

false