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AB109268

Anti-mTOR (phospho S2448) antibody [EPR426(2)]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

4

(6 Reviews)

|

(724 Publications)

Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit monoclonal antibody detecting mTOR in Western Blot, IHC-P, IHC-Fr, Dot Blot. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 510 publications

View Alternative Names

FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR

9 Images
Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

IHC image of mTOR (phospho S2448) staining in a section of frozen normal human heart performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab109268, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab109268 at a dilution of 1/50. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

Immunohistochemical cytoplasmic and nuclear staining of paraffin embedded human endometrium carcinoma with purified ab109268 at a working dilution of 1 in 100. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • WB

Lab

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

All lanes:

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/5000 dilution

Lane 1:

untreated HEK293 cell lysate at 10 µg

Lane 2:

HEK293 cell lysate treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 289 kDa

Observed band size: 289 kDa

false

Exposure time: 3min

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • WB

Lab

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

All lanes:

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution

Lane 1:

untreated HEK293 cell lysate at 10 µg

Lane 2:

HEK293 cell lysate treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 289 kDa

Observed band size: 289 kDa

false

Exposure time: 3min

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • WB

Lab

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

All lanes:

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution

Lane 1:

untreated HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with insulin at 10 µg

Lane 3:

HeLa cell lystae treated with insulin, and the membrane treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 289 kDa

Observed band size: 289 kDa

false

Exposure time: 1min

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • WB

Lab

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

All lanes:

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate at 10 µg

Lane 3:

HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 289 kDa

Observed band size: 289 kDa

false

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • WB

Lab

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

All lanes:

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution

Lane 1:

untreated NIH/3T3 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate treated with insulin at 10 µg

Lane 3:

NIH/3T3 cell lysate treated with insulin, then the membrane treated wth alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 289 kDa

Observed band size: 289 kDa

false

Exposure time: 2min

Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
  • Dot

Lab

Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

Dot blot analysis of mTOR (phospho S2448) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2448) phospho peptide with purified ab109268 at a dilution of 1/1000 (0.073ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.

Blocking buffer : 5% NFDM/TBST
Diluting buffer : 5% NFDM /TBST

  • Carrier free

    Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR426(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IHC-Fr, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Dot Blot in Human, Mouse samples.

What is the molecular weight of mTOR?
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) specifically detects a band for mTOR (UniProt: P42345) at a molecular weight of 289kDa.

Trusted by the scientific community
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) was first used in a scientific publication in 2011 and has been cited over 510 times in peer-reviewed journals.

Reviewed by scientists
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR426(2)] also available for your convenience: ab109268, Carrier free - ab177734

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mammalian target of rapamycin commonly known as mTOR is a serine/threonine kinase known for its role in cellular growth and metabolism. It has a molecular weight of approximately 289 kDa. mTOR is expressed in various tissues throughout the body including muscle adipose tissue and the brain. The protein functions as a central regulator of cell proliferation protein synthesis and nutrient signaling. Often researchers utilize mTOR ELISA or mTOR western blot (mTOR WB) methods and mTOR antibodies to study its expression and activity in various biological contexts.
Biological function summary

MTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.

Pathways

MTOR plays a pivotal role in the PI3K/AKT/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.

MTOR has connections to cancer and neurodegenerative diseases. Its dysregulation often leads to uncontrolled cellular proliferation a hallmark of many cancers. Conditions such as tuberous sclerosis can occur due to mutations in proteins like TSC1 and TSC2 that regulate mTOR activity. In Alzheimer's disease mTOR's role in autophagy and protein synthesis becomes significant as imbalance may contribute to disease progression. Understanding these connections highlights the potential of targeting mTOR pathways therapeutically.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the MTOR gene is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival in response to various signals, such as hormones and nutrients. MTOR operates within two distinct signaling complexes, mTORC1 and mTORC2. mTORC1 is activated to upregulate protein synthesis by phosphorylating regulators of mRNA translation and ribosome synthesis, and phosphorylates and activates proteins like RPS6KB1 and RPS6KB2 to promote protein synthesis. It controls MiT/TFE factors TFEB and TFE3 by mediating their retention and inactivation under nutrient-rich conditions, and it inhibits autophagy by phosphorylating DAP and RUBCNL/Pacer. Additionally, mTORC1 engages in feedback control on growth factor signaling and may influence microtubules through CLIP1 phosphorylation. The mTORC2 complex may regulate cellular processes, including survival and cytoskeletal organization, by phosphorylating AKT1 and regulating the actin cytoskeleton via PRKCA, PXN, and Rho-type guanine nucleotide exchange factors. It also regulates the phosphorylation of SGK1. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MTOR phospho S2448
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Publications (724)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:715 PubMed41057302

2025

Fatty acid synthase-mediated lipid droplet formation enhances macrophage killing of Staphylococcus aureus.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Wu,Jiaxin Shen,Shenwei Gao,Miao Li,Qingyu Weng,Kua Zheng,Chen Zhu,Zhongnan Qin,Jieyu Li,Jiafei Lou,Songmin Ying,Yinfang Wu,Zhihua Chen,Wen Li

American journal of translational research 17:6103-6112 PubMed40950323

2025

TRAIL-mediated armA upregulation enhances the drug resistance of by activating the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bu Wang,Wei Zhang,Jianhua Liu,Hongxia Zhang,Maochen Li,Xiaocui Peng,Zhihua Zhang,Ning Song

Investigative ophthalmology & visual science 66:59 PubMed40856653

2025

Single-Cell Transcriptomics Reveals Effects of Long-Term Contact Lens Wearing on the Human Corneal Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Zhang,Di Sun,Ting Liu,Ning Wang,Shengqian Dou,Qun Wang,Dewei Li,Yi Song,Le Tian,Luqin Wan,Min Chen

Annals of hematology 104:4101-4109 PubMed40825899

2025

Sirolimus modulates the erythroid differentiation of K562 cells by upregulating SHP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yang,Zengwei Tang,Qinglin Hu,Chen Yang,Miao Chen,Hongmei Jing,Bing Han

Scientific reports 15:29560 PubMed40797086

2025

Exosomal LncRNA SCAMP1-AS1 enhances osteosarcoma malignancy by regulating the LKB1-AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Li,Xiuqi Zou,Xiaomin Feng,Jing Xia,Zhifeng Wu,Haili Ma

Reproductive sciences (Thousand Oaks, Calif.) 32:2970-2984 PubMed40789984

2025

Kuntai Capsules Improve Premature Ovarian Failure by Regulating AMPK-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Ye,Miao Chen,Jiajing Zhong,Haofan Chen,Xinmiao Lin

Dose-response : a publication of International Hormesis Society 23:15593258251349653 PubMed40771730

2025

Ginsenoside Rk1 Enhances Chemosensitivity of Gastric Cancer Through Activating the AMPK/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqin Huang,Jian Wu,Li Zheng,Gen Huang,Bin Li,Lihong Gan,Ling Yao

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 29:571-597 PubMed40717511

2025

Mechanisms of Xuefu Zhuyu decoction in treating diabetic kidney disease-induced renal fibrosis: UPLC-Q/TOF-MS, network pharmacology, and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Zhang,Shuaixing Zhang,Zeyu Zhang,Zijing Cao,Xuehui Bai,Shujiao Zhang,Mengqi Zhou,Jingyi Tang,Yiran Xie,Zhongjie Liu,Weijing Liu,Yuning Liu

Cancer gene therapy 32:1008-1017 PubMed40702333

2025

Rapamycin rescues APC-mutated colon organoid differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Aline Habib,Rose Mamistvalov,Dalit Ben-Yosef

Histology and histopathology :18964 PubMed40692330

2025

Lysimachia christinae Hance aqueous extract ameliorates renal injury in kidney stone rats and calcium oxalate crystal-induced oxidative stress in HK-2 cells via inhibiting the PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xintian Zheng,Shengni Lv,Wangen Wang,Liangrong Zhu,Luning Lin
View all publications
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Product promise

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