Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit monoclonal antibody that is used to detect mTOR in Western Blot, IHC-P, IHC-Fr, Dot Blot. Suitable for Human, Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 510 publications
Images
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img21627.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)")
![Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--immunohistochemistry-frozen-sections-img87294.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110472.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)")
![Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--dot-blot-img3346.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)")
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img19412.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)")
Publications
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- MedComm 4:e4332023Integrated transcriptomics, proteomics, and functional analysis to characterize the tissue-specific small extracellular vesicle network of breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLesang Shen,Huanhuan Huang,Zichen Wei,Wuzhen Chen,Jiaxin Li,Yao Yao,Jun Zhou,Jian Liu,Shanshan Sun,Wenjie Xia,Ting Zhang,Xiuyan Yu,Jun Shen,Weilan Wang,Jingxin Jiang,Jian Huang,Ming Jiang,Chao NiPubMed 38053815
- Pharmacology 109:34-422023Impairment of Autophagy Mediates the Uric-Acid-Induced Phenotypic Transformation of Vascular Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Lu,Hanlin Zhang,Min Han,Ping Wang,Liping MengPubMed 38011839
- Cell death & disease 14:7742023Hypoxia-induced PLOD2 promotes clear cell renal cell carcinoma progression via modulating EGFR-dependent AKT pathway activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Liu,Wan Xiang,Zhizhuang Chen,Gang Wang,Rui Cao,Fenfang Zhou,Zhe Meng,Yongwen Luo,Liang ChenPubMed 38008826
- Cell death discovery 9:4252023Peroxiredoxin 1 regulates crosstalk between pyroptosis and autophagy in oral squamous cell carcinoma leading to a potential pro-survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeilin Ye,Ting Liu,Shanshan Liu,Rong Tang,Hongrui Liu,Fan Zhang,Shenglei Luo,Minqi LiPubMed 38007535
- Journal of diabetes research 2023:41933092023High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxiao Liu,Xingna Hu,Yun Zhao,Aijie Huang,Jiaqi Chen,Ting Lu,Mian Wu,Honghong LuPubMed 38033740
- International journal of molecular sciences 24:2023Increased IGFBP2 Levels by Placenta-Derived Mesenchymal Stem Cells Enhance Glucose Metabolism in a TAA-Injured Rat Model via AMPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDae-Hyun Lee,Hyeri Park,Jun-Hyeong You,Jin Seok,Dong-Wook Kwon,Young-Ran Kim,Gi-Jin KimPubMed 38003735
- American journal of cancer research 13:5656-56662023The transcription factor TBP promotes hepatocellular carcinoma progression by activating AKT3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Jiang,Yihuang Lin,Jie Du,Shi Qiu,Gang Luo,Ziqiang ZhangPubMed 38058816
- Polish journal of pathology : official journal of the Polish Society of Pathologists 74:182-1932023S100A16 cooperates with DEPDC1 to promote the progression and angiogenesis of nephroblastoma through PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeng Geng,Yongtao Xu,Qingfang Li,Qinghao Li,Lili Yuan,Mengyao Dong,Ming MingPubMed 37955537
- Immunity, inflammation and disease 11:e10612023Chrysosplenol D can inhibit the growth of prostate cancer by inducing reactive oxygen species and autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoyu Zhang,Zhixin You,Yilei Li,Cheng Gao,Yuhao Wang,Xiaoxiang ZhangPubMed 37904714
- Translational neuroscience 14:202203142023CST3 alleviates bilirubin-induced neurocytes' damage by promoting autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenkun Li,Yating DuPubMed 37854583
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | Dot | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Expected | Tested | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Predicted | Not recommended | Predicted |
| Pig | Predicted | Not recommended | Not recommended | Predicted | Predicted | Not recommended | Predicted |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Pig, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Pig | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Pig, Rat, Synthetic peptide | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
The protein expressed by the MTOR gene is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival in response to various signals, such as hormones and nutrients. MTOR operates within two distinct signaling complexes, mTORC1 and mTORC2. mTORC1 is activated to upregulate protein synthesis by phosphorylating regulators of mRNA translation and ribosome synthesis, and phosphorylates and activates proteins like RPS6KB1 and RPS6KB2 to promote protein synthesis. It controls MiT/TFE factors TFEB and TFE3 by mediating their retention and inactivation under nutrient-rich conditions, and it inhibits autophagy by phosphorylating DAP and RUBCNL/Pacer. Additionally, mTORC1 engages in feedback control on growth factor signaling and may influence microtubules through CLIP1 phosphorylation. The mTORC2 complex may regulate cellular processes, including survival and cytoskeletal organization, by phosphorylating AKT1 and regulating the actin cytoskeleton via PRKCA, PXN, and Rho-type guanine nucleotide exchange factors. It also regulates the phosphorylation of SGK1. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR
Recommended products
Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit monoclonal antibody that is used to detect mTOR in Western Blot, IHC-P, IHC-Fr, Dot Blot. Suitable for Human, Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 510 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR426(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Dot Blot in Human, Mouse samples.
What is the molecular weight of mTOR?
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) specifically detects a band for mTOR (UniProt: P42345) at a molecular weight of 289kDa.
Trusted by the scientific community
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) was first used in a scientific publication in 2011 and has been cited over 510 times in peer-reviewed journals.
Reviewed by scientists
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR426(2)] also available for your convenience:
ab109268, Carrier free - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free ab177734
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mammalian target of rapamycin commonly known as mTOR is a serine/threonine kinase known for its role in cellular growth and metabolism. It has a molecular weight of approximately 289 kDa. mTOR is expressed in various tissues throughout the body including muscle adipose tissue and the brain. The protein functions as a central regulator of cell proliferation protein synthesis and nutrient signaling. Often researchers utilize mTOR ELISA or mTOR western blot (mTOR WB) methods and mTOR antibodies to study its expression and activity in various biological contexts.
- Biological function summary
MTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.
- Pathways
MTOR plays a pivotal role in the PI3K/AKT/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.
- Associated diseases and disorders
MTOR has connections to cancer and neurodegenerative diseases. Its dysregulation often leads to uncontrolled cellular proliferation a hallmark of many cancers. Conditions such as tuberous sclerosis can occur due to mutations in proteins like TSC1 and TSC2 that regulate mTOR activity. In Alzheimer's disease mTOR's role in autophagy and protein synthesis becomes significant as imbalance may contribute to disease progression. Understanding these connections highlights the potential of targeting mTOR pathways therapeutically.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img21627.jpg "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
MTOR (phospho S2448) Western blot staining using rabbit Anti-mTOR (phospho S2448) antibody
All lanes: Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2: HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate at 10 µg
Lane 3: HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
![Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--immunohistochemistry-frozen-sections-img87294.jpg "Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
IHC image of mTOR (phospho S2448) staining in a section of frozen normal human heart performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab109268, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110472.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab109268 at a dilution of 1/50. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--dot-blot-img3346.jpg "Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
Dot blot analysis of mTOR (phospho S2448) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2448) phospho peptide with purified ab109268 at a dilution of 1/1000 (0.073ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/100,000.
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM /TBST![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img19412.jpg "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
MTOR (phospho S2448) Western blot staining using rabbit Anti-mTOR (phospho S2448) antibody
All lanes: Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution
Lane 1: untreated NIH/3T3 cell lysate at 10 µg
Lane 2: NIH/3T3 cell lysate treated with insulin at 10 µg
Lane 3: NIH/3T3 cell lysate treated with insulin, then the membrane treated wth alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 2min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135314.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
Immunohistochemical cytoplasmic and nuclear staining of paraffin embedded human endometrium carcinoma with purified ab109268 at a working dilution of 1 in 100. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img20006.jpg "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
MTOR (phospho S2448) Western blot staining using rabbit Anti-mTOR (phospho S2448) antibody
All lanes: Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution
Lane 1: untreated HeLa cell lysate at 10 µg
Lane 2: HeLa cell lysate treated with insulin at 10 µg
Lane 3: HeLa cell lystae treated with insulin, and the membrane treated with alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 1min
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img41880.jpg "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
MTOR (phospho S2448) Western blot staining using rabbit Anti-mTOR (phospho S2448) antibody
All lanes: Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution
Lane 1: untreated HEK293 cell lysate at 10 µg
Lane 2: HEK293 cell lysate treated with alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 3min
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img21406.jpg "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)
MTOR (phospho S2448) Western blot staining using rabbit Anti-mTOR (phospho S2448) antibody
All lanes: Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/5000 dilution
Lane 1: untreated HEK293 cell lysate at 10 µg
Lane 2: HEK293 cell lysate treated with alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135314.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img20006.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img41880.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
![Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268), expandable thumbnail](https://content.abcam.com/products/images/mtor-phospho-s2448-antibody-epr4262-ab109268--western-blot-img21406.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)")
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