AB137133

Anti-mTOR (phospho S2481) antibody [EPR427(N)]

RabMAb
Recombinant
Lab Essentials
20ul selling size
What is this?

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(116 Publications)

Rabbit Recombinant Monoclonal MTOR phospho S2481 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 116 publications.

View Alternative Names

FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR

3 Images

Lab

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (AB137133)

Blocking Buffer : 5% NFDM/TBST
Diluting Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate at 10 µg

Lane 2:

HeLa grown in serum-free media overnight, then treated with 200 nM PMA for 4 hours, whole cell lysate at 10 µg

Lane 3:

HeLa grown in serum-free media overnight, then treated with 200 nM PMA for 4 hours, whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 289 kDa

Observed band size: 289 kDa

false

Unknown

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (AB137133)

All lanes:

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate, untreated at 10 µg

Lane 2:

HEK-293 cell lysate, treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 289 kDa

false

Lab

Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (AB137133)

Dot blot analysis of mTOR (phospho S2481) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2481) phospho peptide with purified ab137133 at a dilution of 1/1000 (1.21ug/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

Blocking buffer : 5% NFDM/TBST
Diluting buffer : 5% NFDM /TBST

Carrier free

Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR427(N)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mammalian target of rapamycin commonly known as mTOR is a serine/threonine kinase known for its role in cellular growth and metabolism. It has a molecular weight of approximately 289 kDa. mTOR is expressed in various tissues throughout the body including muscle adipose tissue and the brain. The protein functions as a central regulator of cell proliferation protein synthesis and nutrient signaling. Often researchers utilize mTOR ELISA or mTOR western blot (mTOR WB) methods and mTOR antibodies to study its expression and activity in various biological contexts.

Biological function summary

MTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.

Pathways

MTOR plays a pivotal role in the PI3K/AKT/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.

MTOR has connections to cancer and neurodegenerative diseases. Its dysregulation often leads to uncontrolled cellular proliferation a hallmark of many cancers. Conditions such as tuberous sclerosis can occur due to mutations in proteins like TSC1 and TSC2 that regulate mTOR activity. In Alzheimer's disease mTOR's role in autophagy and protein synthesis becomes significant as imbalance may contribute to disease progression. Understanding these connections highlights the potential of targeting mTOR pathways therapeutically.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the MTOR gene is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival in response to various signals, such as hormones and nutrients. MTOR operates within two distinct signaling complexes, mTORC1 and mTORC2. mTORC1 is activated to upregulate protein synthesis by phosphorylating regulators of mRNA translation and ribosome synthesis, and phosphorylates and activates proteins like RPS6KB1 and RPS6KB2 to promote protein synthesis. It controls MiT/TFE factors TFEB and TFE3 by mediating their retention and inactivation under nutrient-rich conditions, and it inhibits autophagy by phosphorylating DAP and RUBCNL/Pacer. Additionally, mTORC1 engages in feedback control on growth factor signaling and may influence microtubules through CLIP1 phosphorylation. The mTORC2 complex may regulate cellular processes, including survival and cytoskeletal organization, by phosphorylating AKT1 and regulating the actin cytoskeleton via PRKCA, PXN, and Rho-type guanine nucleotide exchange factors. It also regulates the phosphorylation of SGK1. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MTOR phospho S2481

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Publications (116)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40897470

2025

Amino acid-dependent TSC2 dephosphorylation by lysosome-PP2A regulates mTORC1 signaling transduction.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Nakamura,Shigeyuki Nada,Masaki Matsumoto,Nuha Loling Othman,Hidetaka Kosako,Kazuki Ikeda,Naohiko Koshikawa,Junya Masumoto,Tatsuya Sawasaki,Mutsuhiro Takekawa,Takashi Suzuki,Masato Okada

Drug design, development and therapy 19:3139-3158 PubMed40291161

2025

Exploring the Therapeutic Mechanism of Jianpi Zhidong Decoction on Tourette Syndrome Based on Proteomics and Network Pharmacology.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Zhang,Hongxian Zhang,Jianning Guo,Yaluan Ma,Xue Bai,Ning Ma,Xiaoxiao Ji,Yanli Meng,Huifang Li,Tananan Sangwanit,Yixin Shi,Jing Zhao,Xiang Li,Jingyuan Lin,Xia Cui

Veterinary sciences 12: PubMed40267013

2025

Exogenous L-Serine Alleviates -Induced Inflammation by Reprogramming the Transcription and Metabolism of Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Fang He,Zhengchun Lang,Yanlan Huang,Yangyang Qiu,Pan Xiong,Nengzhang Li,Guangfu Zhao,Yuanyi Peng

Lipids in health and disease 24:146 PubMed40241082

2025

Radiation induced dermatitis by increasing triglyceride levels to induce autophagy and inhibit the PI3K/Akt/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yafang Hong,Hongdan Guan,Yunhao Chen,Yao Wang,Junjian Lin,Ying Wang,Yang Zhang,Rong Zheng,Xingchen Ding,Zihan Zhou,Benhua Xu

Journal of diabetes research 2025:4656485 PubMed40225010

2025

Revealing the Mechanisms of Shikonin Against Diabetic Wounds: A Combined Network Pharmacology and In Vitro Investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Tian,Junchao Wu,Qian Du,Jiale Han,Meng Yang,Xiang Li,Mingzhu Li,Xiaofeng Ding,Yeqiang Song

Nature 641:427-437 PubMed40205038

2025

Stress dynamically modulates neuronal autophagy to gate depression onset.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yang,Chen Guo,Zhiwei Zheng,Yiyan Dong,Qifeng Xie,Zijian Lv,Min Li,Yangyang Lu,Xiaonan Guo,Rongshan Deng,Yiqin Liu,Yirong Feng,Ruiqi Mu,Xuliang Zhang,Huan Ma,Zhong Chen,Zhijun Zhang,Zhaoqi Dong,Wei Yang,Xiangnan Zhang,Yihui Cui

Open life sciences 19:20221007 PubMed39711976

2024

ZAG promotes colorectal cancer cell proliferation and epithelial-mesenchymal transition by promoting lipid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maotao Xu,Xingzheng Jin,Zhouli Shen

Scientific reports 14:24529 PubMed39424901

2024

Trophoblast cell-derived extracellular vesicles regulate the polarization of decidual macrophages by carrying miR-141-3p in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Dongcai Wu,Bo Zhou,Lan Hong,Hui Cen,Ling Wang,Yanlin Ma,Humin Gong

Molecular medicine reports 30: PubMed39219289

2024

inhibits hepatic fibrosis and hepatic stellate cell activation by targeting the PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiheng Dong,Haibin Guan,Lu Wang,Lijuan Liang,Yifan Zang,Lan Wu,Lidao Bao

Pharmacological reports : PR 76:1001-1011 PubMed39042346

2024

Subchronic administration of scopolamine reverses UCMS-induced behavior in mice via eEF2 protein dephosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yana Babii,Agnieszka Pałucha-Poniewiera,Anna Rafało-Ulińska,Piotr Brański,Andrzej Pilc

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mTOR (phospho S2481) antibody [EPR427(N)]

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (AB137133)

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (AB137133)

Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (AB137133)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (116)

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