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AB154555

Anti-MTPAP antibody

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(2 Publications)

Rabbit Polyclonal MTPAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTPAP aa 1 to C-terminus.

View Alternative Names

PAPD1, MTPAP, PAP, PAP-associated domain-containing protein 1, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, mtPAP, TUTase 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MTPAP antibody (AB154555)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MTPAP antibody (AB154555)

Immunofluorescence analysis of methanol-fixed Hela cell labeling MTPAP, mitochondrial with ab154555 at a 1/200 dilution. The lower panel was costained with Hoechst 33342.

Western blot - Anti-MTPAP antibody (AB154555)
  • WB

Unknown

Western blot - Anti-MTPAP antibody (AB154555)

7.5% SDS PAGE

All lanes:

Western blot - Anti-MTPAP antibody (ab154555) at 1/5000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MTPAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVV4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
See full target information MTPAP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:4689-4704 PubMed33836087

2021

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghai Zhang,Xiao He,Shihao Yao,Tianxiang Lin,Luwen Zhang,Danni Chen,Chao Chen,Qingxian Yang,Feng Li,Yi-Min Zhu,Min-Xin Guan

Nucleic acids research 46:5209-5226 PubMed29518244

2018

Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Toompuu,Tea Tuomela,Pia Laine,Lars Paulin,Eric Dufour,Howard T Jacobs
View all publications
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Product promise

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