Rabbit Polyclonal MTPAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTPAP aa 1 to C-terminus.
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Publications
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- Nucleic acids research 49:4689-47042021Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinghai Zhang,Xiao He,Shihao Yao,Tianxiang Lin,Luwen Zhang,Danni Chen,Chao Chen,Qingxian Yang,Feng Li,Yi-Min Zhu,Min-Xin GuanPubMed 33836087
- Nucleic acids research 46:5209-52262018Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Toompuu,Tea Tuomela,Pia Laine,Lars Paulin,Eric Dufour,Howard T JacobsPubMed 29518244
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MTPAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NVV4
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
Alternative names
PAPD1, MTPAP, PAP, PAP-associated domain-containing protein 1, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, mtPAP, TUTase 1
Recommended products
Rabbit Polyclonal MTPAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTPAP aa 1 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MTPAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NVV4
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-MTPAP antibody (ab154555)
7.5% SDS PAGE
All lanes: Western blot - Anti-MTPAP antibody (ab154555) at 1/5000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MTPAP antibody (ab154555)
Immunofluorescence analysis of methanol-fixed Hela cell labeling MTPAP, mitochondrial with ab154555 at a 1/200 dilution. The lower panel was costained with Hoechst 33342.
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