AB156684

Anti-MTPAP antibody

Name: Anti-MTPAP antibody (ab156684) | Abcam
Brand: Abcam Limited
SKU: AB156684

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(1 Publication)

Rabbit Polyclonal MTPAP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MTPAP aa 500 to C-terminus.

View Alternative Names

PAPD1, MTPAP, PAP, PAP-associated domain-containing protein 1, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, mtPAP, TUTase 1

2 Images

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Immunoprecipitation - Anti-MTPAP antibody (AB156684)

Detection of MTPAP, mitochondrial by Western blot of Immunoprecipitate :
Immunoprecipitation of HeLa whole cell lysate using ab156684 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of MTPAP, mitochondrial with ab156684 at 1μg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MTPAP antibody (ab156684)

Predicted band size: 66 kDa

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Western blot - Anti-MTPAP antibody (AB156684)

All lanes:

Western blot - Anti-MTPAP antibody (ab156684) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 66 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human MTPAP aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVV4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab156684 was affinity purified using an epitope specific to MTPAP, mitochondrial immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.

See full target information MTPAP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5609 PubMed36153345

2022

Human centromere repositioning activates transcription and opens chromatin fibre structure.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Naughton,Covadonga Huidobro,Claudia R Catacchio,Adam Buckle,Graeme R Grimes,Ryu-Suke Nozawa,Stefania Purgato,Mariano Rocchi,Nick Gilbert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTPAP antibody

Immunoprecipitation - Anti-MTPAP antibody (AB156684)

Western blot - Anti-MTPAP antibody (AB156684)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (1)

Product promise