Rabbit Polyclonal MTR4 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MTREX.
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- Clinical and translational medicine 13:e12732023NSUN2-mediated m C RNA methylation dictates retinoblastoma progression through promoting PFAS mRNA stability and expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSipeng Zuo,Lin Li,Xuyang Wen,Xiang Gu,Ai Zhuang,Rui Li,Fuxiang Ye,Shengfang Ge,Xianqun Fan,Jiayan Fan,Peiwei Chai,Linna LuPubMed 37228185
- Journal of advanced research 39:103-1172022miR-155 down-regulation protects the heart from hypoxic damage by activating fructose metabolism in cardiac fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhang,Hong Zhang,Zhan Yang,Xin-Hua Zhang,Qing Miao,Min Li,Tian-Ying Zhai,Bin Zheng,Jin-Kun WenPubMed 35777901
- Molecular cell 82:2505-2518.e72022Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPiotr Gerlach,William Garland,Mahesh Lingaraju,Anna Salerno-Kochan,Fabien Bonneau,Jérôme Basquin,Torben Heick Jensen,Elena ContiPubMed 35688157
- Molecular cell 81:3949-3964.e72021Mechanism of noncoding RNA-associated N-methyladenosine recognition by an RNA processing complex during IgH DNA recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLekha Nair,Wanwei Zhang,Brice Laffleur,Mukesh K Jha,Junghyun Lim,Heather Lee,Lijing Wu,Nehemiah S Alvarez,Zhi-Ping Liu,Emilia L Munteanu,Theresa Swayne,Jacob H Hanna,Lei Ding,Gerson Rothschild,Uttiya BasuPubMed 34450044
- Cancer cell 39:958-972.e82021N-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanming Cheng,Wei Xie,Brian F Pickering,Karen L Chu,Angela M Savino,Xuejing Yang,Hanzhi Luo,Diu Tt Nguyen,Shanlan Mo,Ersilia Barin,Anthony Velleca,Thomas M Rohwetter,Dinshaw J Patel,Samie R Jaffrey,Michael G KharasPubMed 34048709
- Frontiers in cell and developmental biology 9:6332592021SYVN1-MTR4-MAT2A Signaling Axis Regulates Methionine Metabolism in Glioma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLude Wang,Bin Hu,Kailing Pan,Jie Chang,Xiaoya Zhao,Lin Chen,Haiping Lin,Jing Wang,Gezhi Zhou,Wenxia Xu,Jianlie YuanPubMed 33859984
- Journal of virology 94:2020Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulio C Ruiz et. al.PubMed 32376621
- Nucleic acids research 48:3869-38872020Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlin Hou,Yuanjie Wei,Yingying Lin,Xiwei Wang,Yiwei Lai,Menghui Yin,Yanpu Chen,Xiangpeng Guo,Senbin Wu,Yindi Zhu,Jie Yuan,Muqddas Tariq,Na Li,Hao Sun,Huating Wang,Xiaofei Zhang,Jiekai Chen,Xichen Bao,Ralf JauchPubMed 32016422
- Nature communications 11:7082020MTR4 drives liver tumorigenesis by promoting cancer metabolic switch through alternative splicing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Yu et. al.PubMed 32024842
- Methods in molecular biology (Clifton, N.J.) 2062:127-1452019Global Identification of Human Exosome Substrates Using RNA Interference and RNA Sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Lloret-Llinares,Torben Heick JensenPubMed 31768975
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MTREX. The exact immunogen used to generate this antibody is proprietary information. Database link P42285
Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Epitope retrieval with Tris-EDTA pH9.0 is recommended for formalin-fixed paraffin-embedded cells. |
Species Human | Dilution info - | Notes Epitope retrieval with Tris-EDTA pH9.0 is recommended for formalin-fixed paraffin-embedded cells. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the ATP-dependent unwinding of RNA duplexes with a single-stranded 3' RNA extension (PubMed:27871484, PubMed:29844170, PubMed:29906447). Central subunit of many protein complexes, namely TRAMP-like, nuclear exosome targeting (NEXT) and poly(A) tail exosome targeting (PAXT) (PubMed:21855801, PubMed:27871484, PubMed:29844170). NEXT functions as an RNA exosome cofactor that directs a subset of non-coding short-lived RNAs for exosomal degradation. NEXT is involved in surveillance and turnover of aberrant transcripts and non-coding RNAs (PubMed:27871484, PubMed:29844170). PAXT directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor ZCCHC8, which links to RNA-binding protein adapters (PubMed:27871484). Associated with the RNA exosome complex and involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA (PubMed:17412707, PubMed:29107693). May be involved in pre-mRNA splicing. In the context of NEXT complex can also in vitro unwind DNA:RNA heteroduplexes with a 3' poly (A) RNA tracking strand (PubMed:29844170). Can promote unwinding and degradation of structured RNA substrates when associated with the nuclear exosome and its cofactors. Can displace a DNA strand while translocating on RNA to ultimately degrade the RNA within a DNA/RNA heteroduplex (PubMed:29906447). Plays a role in DNA damage response (PubMed:29902117).
Alternative names
DOB1, KIAA0052, MTR4, SKIV2L2, MTREX, Exosome RNA helicase MTR4, ATP-dependent RNA helicase DOB1, ATP-dependent RNA helicase SKIV2L2, Superkiller viralicidic activity 2-like 2, TRAMP-like complex helicase
Recommended products
Rabbit Polyclonal MTR4 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MTREX.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MTREX. The exact immunogen used to generate this antibody is proprietary information. Database link P42285
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling MTR4 with ab70551 at 1/200 (1μg/ml). Detection: DAB.
Immunoprecipitation - Anti-MTR4 antibody (ab70551)
Detection of human MTR4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTR4 antibody ab70551 used for IP at 3 μg per reaction. MTR4 was also immunoprecipitated by rabbit anti-MTR4 antibody Anti-MTR4 antibody ab70552. For blotting immunoprecipitated MTR4, ab70551 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-MTR4 antibody (ab70551)
Predicted band size: 118 kDa
Western blot - Anti-MTR4 antibody (ab70551)
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Western blot - Anti-MTR4 antibody (ab70551) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Predicted band size: 118 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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