AB119684

Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker

Name: Anti-mtTFA antibody [18G102B2E11] Mouse monoclonal (ab119684) | Abcam
Brand: Abcam Limited
SKU: AB119684
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(1 Review)

(36 Publications)

Mouse Monoclonal mtTFA antibody. Mitochondrion marker. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Recombinant full length protein samples. Cited in 36 publications.

View Alternative Names

TCF6, TCF6L2, TFAM, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2, MtTF1, TCF-6

4 Images

Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

Flow Cyt

Unknown

Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

IHC image of mtTFA staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119684, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.

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Western blot - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

All lanes:

Western blot - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684) at 1 µg/mL

Lane 1:

recombinant mtTFA (<a href='/en-us/products/proteins-peptides/recombinant-human-mttfa-protein-ab103784'>ab103784</a>) at 0.025 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution

Predicted band size: 29 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

18G102B2E11

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Recombinant full length protein": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

Purity is near homogeneity as judged by SDS-PAGE. ab119684 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.

Biological function summary

MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.

Pathways

The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.

MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed : 29445193, PubMed : 32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed : 22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed : 22037172). Is able to unwind DNA (PubMed : 22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed : 1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed : 19304746, PubMed : 22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed : 22037171).

See full target information TFAM

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:1570-1592 PubMed40691304

2025

LONP1 regulation of mitochondrial protein folding provides insight into beta cell failure in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Li,Yamei Deng,Marie Gasser,Jie Zhu,Emily M Walker,Vaibhav Sidarala,Emma C Reck,Dre L Hubers,Mabelle B Pasmooij,Chun-Shik Shin,Khushdeep Bandesh,Eftyhmios Motakis,Siddhi Nargund,Romy Kursawe,Venkatesha Basrur,Alexey I Nesvizhskii,Michael L Stitzel,David C Chan,Guy A Rutter,Scott A Soleimanpour

NPJ systems biology and applications 11:67 PubMed40593928

2025

Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Te Lin,Yi-Ju Lee,Wen-Wei Tseng,Zih-Hua Chen,Huai-Ching Hsieh,Ko-Hong Lin,Jin-Yu Su,An-Chi Wei

The EMBO journal 44:1724-1748 PubMed39934413

2025

Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Wang,Tirawit Meerod,Nuria Cortes-Silva,Ason C-Y Chiang,Ziyan Nie,Ying Di,Peiqiang Mu,Ankit Verma,Adam James Reid,Hansong Ma

Molecular biology of the cell 36:ar11 PubMed39705374

2024

MRE11-independent effects of Mirin on mitochondrial DNA integrity and cellular immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Koit Aasumets,Anu Hangas,Georgios Fragkoulis,Cyrielle P J Bader,Direnis Erdinc,Sjoerd Wanrooij,Paulina H Wanrooij,Steffi Goffart,Jaakko L O Pohjoismäki

Biochimica et biophysica acta. Molecular basis of disease 1870:167131 PubMed38521420

2024

A stagewise response to mitochondrial dysfunction in mitochondrial DNA maintenance disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Vincent,Chun Chen,Tiago Bernardino Gomes,Valeria Di Leo,Tuomas Laalo,Kamil Pabis,Rodrick Capaldi,Michael F Marusich,David McDonald,Andrew Filby,Andrew Fuller,Diana Lehmann Urban,Stephan Zierz,Marcus Deschauer,Doug Turnbull,Amy K Reeve,Conor Lawless

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2307136 PubMed38445970

2024

Hallmark Molecular and Pathological Features of POLG Disease are Recapitulated in Cerebral Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Anbin Chen,Tsering Yangzom,Yu Hong,Bjørn Christian Lundberg,Gareth John Sullivan,Charalampos Tzoulis,Laurence A Bindoff,Kristina Xiao Liang

Nature genetics 55:1632-1639 PubMed37723262

2023

Molecular basis for maternal inheritance of human mitochondrial DNA.

Applications

Unspecified application

Species

Unspecified reactive species

William Lee,Angelica Zamudio-Ochoa,Gina Buchel,Petar Podlesniy,Nuria Marti Gutierrez,Margalida Puigròs,Anna Calderon,Hsin-Yao Tang,Li Li,Aleksei Mikhalchenko,Amy Koski,Ramon Trullas,Shoukhrat Mitalipov,Dmitry Temiakov

NPJ Parkinson's disease 9:120 PubMed37553379

2023

Parkinson's disease neurons exhibit alterations in mitochondrial quality control proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chen,David McDonald,Alasdair Blain,Emily Mossman,Kiera Atkin,Michael F Marusich,Roderick Capaldi,Laura Bone,Anna Smith,Andrew Filby,Daniel Erskine,Oliver Russell,Gavin Hudson,Amy E Vincent,Amy K Reeve

International journal of pharmaceutics 636:122808 PubMed36889415

2023

Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Eloy Pena-Rodríguez,Teresa García-Berrocoso,Ezequiel Vázquez Fernández,Francisco J Otero-Espinar,Joaquin Abian,Francisco Fernández-Campos

Nature 614:767-773 PubMed36755096

2023

Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis.

Applications

Unspecified application

Species

Unspecified reactive species

Joe Nassour,Lucia Gutierrez Aguiar,Adriana Correia,Tobias T Schmidt,Laura Mainz,Sara Przetocka,Candy Haggblom,Nimesha Tadepalle,April Williams,Maxim N Shokhirev,Semih C Akincilar,Vinay Tergaonkar,Gerald S Shadel,Jan Karlseder

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker

Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

Western blot - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (AB119684)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

Product promise