Rabbit Recombinant Monoclonal mtTFA antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples.
Images
![Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunoprecipitation-img53069.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35245.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958)")
![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunocytochemistry-immunofluorescence-img15413.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54785.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958)")
![Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunoprecipitation-img74348.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (AB240958)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171).
Alternative names
TCF6, TCF6L2, TFAM, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2, MtTF1, TCF-6
Recommended products
Rabbit Recombinant Monoclonal mtTFA antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12285
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240958 is the carrier-free version of Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.
- Biological function summary
MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.
- Pathways
The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.
- Associated diseases and disorders
MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunoprecipitation-img53069.jpg "Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 (purified) at 1/40 immunoprecipitating mtTFA in 10 ?g HEK293 (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558)
All lanes: Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558)
Predicted band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35245.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558).
![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunocytochemistry-immunofluorescence-img15413.jpg "Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Immunofluorescence staining of MCF7 cells with purified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.
The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500.
For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54785.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling mtTFA using unpurified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 at a 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunoprecipitation-img74348.jpg "Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Western blot analysis on Immunoprecipitation pellet from either 1) 293T cell lysate, or 2) 1xPBS (negative control), showing mtTFA, using unpurified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558).
All lanes: Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558)
Developed using the ECL technique.
Predicted band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling mtTFA using unpurified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 at a 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunocytochemistry-immunofluorescence-img75362.jpg "Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)
Immunofluorescence analysis of MCF7 cells, labeling mtTFA using unpurified Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab176558).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958), expandable thumbnail](https://content.abcam.com/products/images/mttfa-antibody-epr12285-bsa-and-azide-free-ab240958--immunocytochemistry-immunofluorescence-img75362.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)")
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