AB131607

Anti-mtTFA antibody - Mitochondrial Marker

Name: Anti-mtTFA antibody - Mitochondrial Marker (ab131607) | Abcam
Brand: Abcam Limited
SKU: AB131607
Rating: 4 (7 reviews)

Lab Essentials
What is this?

(7 Reviews)

(182 Publications)

Rabbit Polyclonal mtTFA antibody. Mitochondrion marker. Suitable for WB and reacts with Rat samples. Cited in 182 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tfam.

View Alternative Names

mtTFA, Tfam

2 Images

AbReview46883****

Western blot - Anti-mtTFA antibody - Mitochondrial Marker (AB131607)

All lanes:

Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution

Lane 1:

Mouse liver whole tissue lysate at 25 µg

Lane 2:

Mouse hepatocytes whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

Predicted band size: 29 kDa

true

Exposure time: 10s

Unknown

Western blot - Anti-mtTFA antibody - Mitochondrial Marker (AB131607)

All lanes:

Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution

All lanes:

Rat kidney lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Tfam.

P40630

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.

Biological function summary

MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.

Pathways

The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.

MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform Mitochondrial. Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA (By similarity).. Isoform Nuclear. May also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.

See full target information Tfam

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Publications (182)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 17:1632877 PubMed40900992

2025

Accelerated midlife endocrine and bioenergetic brain aging in APOE4 females.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Wang,Zisu Mao,Yuan Shang,Simona Merlini,Francesca Vitali,Jean-Paul Wiegand,Roberta Diaz Brinton

eLife 14: PubMed40587199

2025

Tissue-specific responses to TFAM and mtDNA copy number manipulation in prematurely ageing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Sophie Kremer,Guanbin Gao,Giovanni Rigoni,Roberta Filograna,Mara Mennuni,Rolf Wibom,Ákos Végvári,Camilla Koolmeister,Nils-Göran Larsson

Nature communications 16:4782 PubMed40404629

2025

Modelling POLG mutations in mice unravels a critical role of POLγΒ in regulating phenotypic severity.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha Corrà,Alessandro Zuppardo,Sebastian Valenzuela,Louise Jenninger,Raffaele Cerutti,Sirelin Sillamaa,Emily Hoberg,Katarina A S Johansson,Urska Rovsnik,Sara Volta,Pedro Silva-Pinheiro,Hannah Davis,Aleksandra Trifunovic,Michal Minczuk,Claes M Gustafsson,Anu Suomalainen,Massimo Zeviani,Bertil Macao,Xuefeng Zhu,Maria Falkenberg,Carlo Viscomi

Journal of nanobiotechnology 23:114 PubMed39962465

2025

RGD hydrogel-loaded ADSC extracellular vesicles mitigate uranium-induced renal injury via TLR4/NF-κB pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Chun-Mei Dai,Bin Zhang,Wan-Xin Zhang,Zheng-Hong Huang,Jiu-Yi Jiang,Shi-Qi Hu,Jia-Hua Ma,Jia-Fu Feng

The Journal of biological chemistry 301:108297 PubMed39947473

2025

Leucine 7 is a key residue for mutant huntingtin-induced mitochondrial pathology and neurotoxicity in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shengrong Zhang,Shengda Wang,Zeyue Yang,Yuanbo Li,Jinping Li,Xushen Chen,Hao Yao,Zhilong Zheng,Xing Guo

Molecular metabolism 93:102107 PubMed39909188

2025

HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Michio Sato,Tsuyoshi Kadomatsu,Jun Morinaga,Yuya Kinoshita,Daisuke Torigoe,Haruki Horiguchi,Sumio Ohtsuki,Shuji Yamamura,Ryoko Kusaba,Takanori Yamaguchi,Goro Yoshioka,Kimi Araki,Tomohiko Wakayama,Keishi Miyata,Koichi Node,Yuichi Oike

Nature communications 16:212 PubMed39747079

2025

Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yi,Guoqiang Wang,Wenhua Zhang,Shuhan Yu,Junjie Fei,Tingting An,Jianqiao Yi,Fengtian Li,Ting Huang,Jian Yang,Mengmeng Niu,Yang Wang,Chuan Xu,Zhi-Xiong Jim Xiao

JCI insight 9: PubMed39704174

2024

RTN1A mediates diabetes-induced AKI-to-CKD transition.

Applications

Unspecified application

Species

Unspecified reactive species

Lulin Min,Ya Chen,Yixin Chen,Fang Zhong,Zhaohui Ni,Leyi Gu,Kyung Lee,John Cijiang He

JCI insight 10: PubMed39589791

2024

Role of cGAS/STING pathway in aging and sexual dimorphism in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sherif Khedr,Lashodya V Dissanayake,Ammar J Alsheikh,Adrian Zietara,Denisha R Spires,Romica Kerketta,Angela J Mathison,Raul Urrutia,Oleg Palygin,Alexander Staruschenko

Frontiers in physiology 15:1420276 PubMed39282091

2024

Temporal expression of mitochondrial life cycle markers during acute and chronic overload of rat plantaris muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Jon-Philippe K Hyatt,Emilie J Lu,Gary E McCall

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-mtTFA antibody - Mitochondrial Marker

Western blot - Anti-mtTFA antibody - Mitochondrial Marker (AB131607)

Western blot - Anti-mtTFA antibody - Mitochondrial Marker (AB131607)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity data

Properties and storage information

Form

Purity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (182)

Product promise