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Rabbit Polyclonal mtTFA antibody. Mitochondrion marker. Suitable for WB and reacts with Rat samples. Cited in 158 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tfam.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Standard buffer

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Mouse Tfam. Database link P40630

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Rat
Tested

Tested
Tested

Species

Rat

Dilution info

1/2000

Notes

-

Alternative names

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Rabbit Polyclonal mtTFA antibody. Mitochondrion marker. Suitable for WB and reacts with Rat samples. Cited in 158 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tfam.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Mouse Tfam. Database link P40630
Purity

Whole antiserum

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.

Activity summary

MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.

Pathways

The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.

Associated diseases and disorders

MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.

Product promise

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2 product images

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607), expandable thumbnail

    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)

    All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (AB131607) at 1/2000 dilution

    Lane 1: Mouse liver whole tissue lysate at 25 µg

    Lane 2: Mouse hepatocytes whole cell lysate at 25 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa

    Exposure time: 10s

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607), expandable thumbnail

    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)

    All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (AB131607) at 1/2000 dilution

    All lanes: Rat kidney lysate

    Predicted band size: 29 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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