Rabbit Polyclonal mtTFA antibody. Mitochondrion marker. Suitable for WB and reacts with Rat samples. Cited in 158 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tfam.
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- Antioxidants (Basel, Switzerland) 12:2023Lactate Mediates High-Intensity Interval Training-Induced Promotion of Hippocampal Mitochondrial Function through the GPR81-ERK1/2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinghui Shang,Xuepeng Bian,Lutao Zhu,Jun Liu,Min Wu,Shujie LouPubMed 38136207
- Life science alliance 7:2023Mitochondrial phosphoproteomes are functionally specialized across tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFynn M Hansen,Laura S Kremer,Ozge Karayel,Isabell Bludau,Nils-Göran Larsson,Inge Kühl,Matthias MannPubMed 37984987
- The journal of physiological sciences : JPS 73:272023Emerin deficiency does not exacerbate cardiomyopathy in a murine model of Emery-Dreifuss muscular dystrophy caused by an LMNA gene mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEiji Wada,Kohei Matsumoto,Nao Susumu,Megumi Kato,Yukiko K HayashiPubMed 37940872
- Diabetologia 67:137-1552023Adjudin improves beta cell maturation, hepatic glucose uptake and glucose homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLipeng Ren,Jérémie Charbord,Lianhe Chu,Aurino M Kemas,Maria Bertuzzi,Jiarui Mi,Chen Xing,Volker M Lauschke,Olov AnderssonPubMed 37843554
- Current research in neurobiology 5:1001122023The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChantal A Pileggi,Gaganvir Parmar,Hussein Elkhatib,Corina M Stewart,Irina Alecu,Marceline Côté,Steffany A L Bennett,Jagdeep K Sandhu,Miroslava Cuperlovic-Culf,Mary-Ellen HarperPubMed 38020812
- Cells 12:2023Mitochondrial Dysfunction and Decreased Cytochrome in Cell and Animal Models of Machado-Joseph Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilipa Almeida,Ildete L Ferreira,Luana Naia,Daniela Marinho,Ana Catarina Vilaça-Ferreira,Marta D Costa,Sara Duarte-Silva,Patrícia Maciel,A Cristina RegoPubMed 37830611
- Cells 12:2023LRP1 Deficiency Promotes Mitostasis in Response to Oxidative Stress: Implications for Mitochondrial Targeting after Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGopal V Velmurugan,W Brad Hubbard,Paresh Prajapati,Hemendra J Vekaria,Samir P Patel,Alexander G Rabchevsky,Patrick G SullivanPubMed 37408279
- Chinese medicine 18:322023BaoShenTongLuo formula protects against podocyte injury by regulating AMPK-mediated mitochondrial biogenesis in diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Guo,Mengdi Wang,Yufei Liu,Yanyu Pang,Lei Tian,Jingwen Zhao,Mengchao Liu,Cun Shen,Yuan Meng,Yuefen Wang,Zhen Cai,Wenjing ZhaoPubMed 36967383
- Nature metabolism 5:445-4652023Loss of fatty acid degradation by astrocytic mitochondria triggers neuroinflammation and neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYashi Mi,Guoyuan Qi,Francesca Vitali,Yuan Shang,Adam C Raikes,Tian Wang,Yan Jin,Roberta D Brinton,Haiwei Gu,Fei YinPubMed 36959514
- The EMBO journal 42:e1130332023A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Cao,Jing Zheng,Huayun Wan,Yuqiu Sun,Song Fu,Shanshan Liu,Baiyu He,Gaihong Cai,Yang Cao,Huanwei Huang,Qi Li,Yan Ma,She Chen,Fengchao Wang,Hui JiangPubMed 36896912
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Tfam. Database link P40630
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Reactivity data
Select an application| WB | |
|---|---|
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 | Notes - |
Target data
Function
Isoform Mitochondrial. Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA (By similarity). Isoform Nuclear. May also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.
Alternative names
mtTFA, Tfam
Recommended products
Rabbit Polyclonal mtTFA antibody. Mitochondrion marker. Suitable for WB and reacts with Rat samples. Cited in 158 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tfam.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Tfam. Database link P40630
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.
- Biological function summary
MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.
- Pathways
The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.
- Associated diseases and disorders
MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution
Lane 1: Mouse liver whole tissue lysate at 25 µg
Lane 2: Mouse hepatocytes whole cell lysate at 25 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Exposure time: 10s
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution
All lanes: Rat kidney lysate
Predicted band size: 29 kDa
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