Rabbit Polyclonal mtTFA antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TFAM.
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- Theranostics 14:56-742024Selective induction of Rab9-dependent alternative mitophagy using a synthetic derivative of isoquinoline alleviates mitochondrial dysfunction and cognitive deficits in Alzheimer's disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJee-Hyun Um,Dong Jin Shin,Se Myeong Choi,Alen Benhur Pravin Nathan,Young Yeon Kim,Da Ye Lee,Dae Jin Jeong,Dong Hyun Kim,Kyung Hwa Kim,Young Hye Kim,Jihoon Nah,Jeong-Hee Jeong,Eunhee Yoo,Hwa Kyoung Shin,Hwan Tae Park,Jihoon Jo,Jong Hyun Cho,Jeanho YunPubMed 38164158
- Redox biology 60:1026312023Peroxiredoxin 2 is required for the redox mediated adaptation to exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Xia,Jose C Casas-Martinez,Eduardo Zarzuela,Javier Muñoz,Antonio Miranda-Vizuete,Katarzyna Goljanek-Whysall,Brian McDonaghPubMed 36791646
- Communications biology 5:10882022The purine metabolite inosine monophosphate accelerates myelopoiesis and acute pancreatitis progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Min Luo,Sin Man Lam,Yuan Dong,Xiao-Juan Ma,Cen Yan,Yue-Jie Zhang,Yu Cao,Li Su,Guotao Lu,Jin-Kui Yang,Guanghou Shui,Ying-Mei FengPubMed 36224248
- Renal failure 44:1545-15572022Activation of AMPK-PGC-1α pathway ameliorates peritoneal dialysis related peritoneal fibrosis in mice by enhancing mitochondrial biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wu,Jushuang Li,Baohong Feng,Zhimin Bi,Geli Zhu,Yanxia Zhang,Xiangyou LiPubMed 36148521
- Clinical and translational medicine 11:e2952021Milk fat globule EGF factor 8 restores mitochondrial function via integrin-medicated activation of the FAK-STAT3 signaling pathway in acute pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Ren,Wuming Liu,Lin Zhang,Jia Zhang,Jianbin Bi,Tao Wang,Mengzhou Wang,Zhaoqing Du,Yawen Wang,Lin Zhang,Zheng Wu,Yi Lv,Lingzhong Meng,Rongqian WuPubMed 33634976
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TFAM. Database link Q00059
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171).
Alternative names
TCF6, TCF6L2, TFAM, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2, MtTF1, TCF-6
Recommended products
Rabbit Polyclonal mtTFA antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TFAM.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TFAM. Database link Q00059
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.
- Biological function summary
MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.
- Pathways
The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.
- Associated diseases and disorders
MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Western blot staining using rabbit Anti-mtTFA antibody
12% SDS-PAGE
All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 29 kDa
Immunoprecipitation - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
Immunoprecipitation - Anti-mtTFA antibody (ab272885).Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
A. 40 μg HEK-293T whole cell lysate.
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of mtTFA protein by 2 μg of ab272885
12% SDS-PAGE
The immunoprecipitated mtTFA protein was detected by ab272885 diluted at 1:1000, followed by anti-rabbit IgG-HRP secondary antibody.
All lanes: Immunoprecipitation - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-mtTFA antibody
Paraffin-embedded human breast cancer tissue stained for mtTFA using ab272885 at 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-mtTFA antibody
4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for mtTFA using ab272885 at 1/500 dilution in ICC/IF. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Western blot staining of Neuro-2a (mouse neuroblastoma cell line) whole cell lysate using rabbit Anti-mtTFA antibody
12% SDS-PAGE
All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885) at 1/1000 dilution
All lanes: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Predicted band size: 29 kDa
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Western blot staining using rabbit Anti-mtTFA antibody
12% SDS-PAGE
All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885) at 1/500 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 29 kDa
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-mtTFA antibody
2% paraformaldehyde/culture medium fixed NTERA-2 cl.D1 [NT2/D1] (Human malignant pluripotent embryonic carcinoma cell line) cells stained for mtTFA using ab272885 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342 staining.
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885)
MtTFA Western blot staining using rabbit Anti-mtTFA antibody
12% SDS-PAGE
All lanes: Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab272885) at 1/3000 dilution
Lane 1: Non-transfected HEK-293T whole cell extracts at 30 µg
Lane 2: mTFA shRNA transfected HEK-293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 29 kDa
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