AB307302

Anti-mtTFA antibody [RM1035] - Mitochondrial Marker

Name: Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) | Abcam
Brand: Abcam Limited
SKU: AB307302
Price: 185 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Anti-mtTFA antibody [RM1035] (ab307302) is a rabbit recombinant multiclonal antibody detecting mtTFA in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

TCF6, TCF6L2, TFAM, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2, MtTF1, TCF-6, Hmgts, Tfam, mtTFA, Testis-specific high mobility group protein, TS-HMG

9 Images

Supplier Data

Immunohistochemistry - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling mtTFA with ab307302 at 1/1000 (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mitochondria in human lung carcinoma. The section was incubated with ab307302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Supplier Data

Immunohistochemistry - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling mtTFA with ab307302 at 1/1000 (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mitochondria in human colon. The section was incubated with ab307302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labeling mtTFA with ab307302 at 1/100 (5.4 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing mitochondrial staining in U-2 OS cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab197491 Anti-COX IV Mouse monoclonal antibody (Alexa Fluor® 647) - Mitochondrial Marker was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling mtTFA with ab307302 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

mtTFA was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10µg with ab307302 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307302 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 : ab307302 IP in MCF7 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307302 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg

Lane 2:

MCF7 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 25 kDa

false

Exposure time: 8s

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labeling mtTFA with ab307302 at 1/100 (5.4 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling mtTFA with ab307302 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

mtTFA was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) whole cell lysate 10µg with ab307302 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307302 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : C2C12 (mouse myoblast) whole cell lysate 10µg
Lane 2 : ab307302 IP in C2C12 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307302 in C2C12 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast) whole cell lysate 10 µg

Lane 2:

C2C12 whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab307302 in C2C12 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 25 kDa

false

Exposure time: 24s

Supplier Data

Western blot - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 70 seconds

All lanes:

Western blot - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) non-mitochondrial fraction 20 μg

Lane 2:

HepG2 mitochondria fraction 20 μg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Lane 5:

C2C12 (mouse myoblast) whole cell lysate 20 μg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

Lane 7:

C6 (rat glial tumor glial cell) whole cell lysate 20 μg

Lane 8:

Mouse heart lysate 20 μg

Lane 9:

Rat heart lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 70s

Carrier free

Anti-mtTFA antibody [RM1035] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1035

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt, IHC-P, ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

IHC-P is not suitable for testing mouse and rat samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "IHC-P is not suitable for testing mouse and rat samples.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "IHC-P is not suitable for testing mouse and rat samples.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-mtTFA antibody [RM1035] (ab307302) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.

Biological function summary

MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.

Pathways

The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.

MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed : 29445193, PubMed : 32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed : 22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed : 22037172). Is able to unwind DNA (PubMed : 22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed : 1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed : 19304746, PubMed : 22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed : 22037171).

See full target information TFAM

Additional targets

Tfam

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cancer research and clinical oncology 151:167 PubMed40372538

2025

High-dose vitamin C promotes mitochondrial biogenesis in HCT116 colorectal cancer cells by regulating the AMPK/PGC-1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

RuiYang Hong,Su Min,Jia Huang,Mou Zou,DongYu Zhou,Yun Liang

Journal of toxicologic pathology 38:17-26 PubMed39839726

2025

The role of mitochondrial reactive oxygen species in initiating mitochondrial damage and inflammation in wasp-venom-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lingya Xia,Hai Yuan,Zhao Gao,Ying Lv,Liang Xu,Fengqi Hu

eLife 12: PubMed37505079

2023

Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Enrico Radaelli,Charles-Antoine Assenmacher,Jillian Verrelle,Esha Banerjee,Florence Manero,Salim Khiati,Anais Girona,Guillermo Lopez-Lluch,Placido Navas,Marco Spinazzi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mtTFA antibody [RM1035] - Mitochondrial Marker

Immunohistochemistry - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunohistochemistry - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Western blot - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

Product promise