Name: Anti-mtTFA antibody [RM1035] - Mitochondrial Marker
SKU: ab307302
Rating: 5 (1 reviews)

Anti-mtTFA antibody [RM1035] (ab307302) is a rabbit recombinant multiclonal antibody that is used to detect mtTFA in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Images

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (AB307302), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	ICC/IF	IHC-P	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Not recommended	Tested
Rat	Expected	Expected	Expected	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes IHC-P is not suitable for testing mouse and rat samples.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes IHC-P is not suitable for testing mouse and rat samples.
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Target data

See full target information TFAM

Function

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171).

Additional Targets

Tfam

Alternative names

TCF6, TCF6L2, TFAM, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2, MtTF1, TCF-6

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1035
Purification technique: Affinity purification Protein A
Specificity: IHC-P is not suitable for testing mouse and rat samples.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-mtTFA antibody [RM1035] (ab307302) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MtTFA also known as mitochondrial transcription factor A or TFAM is an important element in mitochondrial DNA regulation. It plays a pivotal role in maintaining mitochondrial genome integrity and ensuring proper mitochondrial function. This protein weighing approximately 25 kDa is expressed within the mitochondria. It binds to the mitochondrial DNA facilitating the transcription and replication processes ensuring the correct functioning of cellular energy metabolism.

Biological function summary

MtTFA is essential for mitochondrial DNA packaging and transcriptional activation. It acts as a binding factor that organizes the mtDNA into a nucleoid-like structure providing protection and stability. MtTFA functions as part of a larger multi-protein complex that includes mitochondrial RNA polymerase ensuring proper transcription of mitochondrial genes. These activities are critical for normal energy production and mitochondrial biogenesis.

Pathways

The mtTFA protein is integral to the oxidative phosphorylation and mitochondrial biogenesis pathways. It orchestrates the expression of genes critical for these pathways alongside related proteins like NRF2 and PGC-1α. MtTFA ensures effective mitochondrial function by modulating the transcription of key mitochondrial-encoded components necessary for electron transport and ATP synthesis thereby sustaining cellular energy balance.

Associated diseases and disorders

MtTFA dysregulation is associated with mitochondrial diseases and neurodegenerative disorders. Abnormal mtTFA activity disrupts mitochondrial DNA maintenance potentially leading to conditions like mitochondrial myopathy. It is also linked with proteins such as POLG which are involved in mitochondrial DNA replication. Understanding mtTFA's role in these pathologies highlights its importance as a target in therapeutic strategies aimed at restoring mitochondrial function and preventing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Immunocytochemistry/ Immunofluorescence staining of C2C12 (mouse myoblasts myoblast) using rabbit Anti-mtTFA antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labeling mtTFA with ab307302 at 1/100 (5.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Immunocytochemistry/ Immunofluorescence staining of U-2 OS (human bone osteosarcoma epithelial cell) using rabbit Anti-mtTFA antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labeling mtTFA with ab307302 at 1/100 (5.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing mitochondrial staining in U-2 OS cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab197491 Anti-COX IV Mouse monoclonal antibody (Alexa Fluor? 647) - Mitochondrial Marker was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Immunohistochemistry staining of Human colon using rabbit Anti-mtTFA antibody
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling mtTFA with ab307302 at 1/1000 (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection). Positive staining on mitochondria in human colon. The section was incubated with ab307302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Immunohistochemistry staining of Human lung carcinoma using rabbit Anti-mtTFA antibody
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling mtTFA with ab307302 at 1/1000 (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection). Positive staining on mitochondria in human lung carcinoma. The section was incubated with ab307302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Flow Cytometry (Intracellular) staining of C2C12 (mouse myoblast) using rabbit Anti-mtTFA antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling mtTFA with ab307302 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Flow Cytometry (Intracellular) staining of MCF7 (human breast adenocarcinoma epithelial cell) using rabbit Anti-mtTFA antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling mtTFA with ab307302 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
mtTFA was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) whole cell lysate 10?g with ab307302 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307302 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: C2C12 (mouse myoblast) whole cell lysate 10?g
Lane 2: abAB307302 IP in C2C12 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307302 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
All lanes: Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) whole cell lysate 10 µg
Lane 2: C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307302 in C2C12 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 25 kDa
Exposure time: 24s
Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
mtTFA was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10?g with ab307302 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307302 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10?g
Lane 2: abAB307302 IP in MCF7 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307302 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: MCF7 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 25 kDa
Exposure time: 8s
Western blot - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302)
MtTFA Western blot staining using rabbit Anti-mtTFA antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 70 seconds
All lanes: Western blot - Anti-mtTFA antibody [RM1035] - Mitochondrial Marker (ab307302) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) non-mitochondrial fraction 20 μg
Lane 2: HepG2 mitochondria fraction 20 μg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 5: C2C12 (mouse myoblast) whole cell lysate 20 μg
Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg
Lane 7: C6 (rat glial tumor glial cell) whole cell lysate 20 μg
Lane 8: Mouse heart lysate 20 μg
Lane 9: Rat heart lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 25 kDa
Exposure time: 70s