AB63467

Anti-MTTP/MTP antibody

(1 Review)

(7 Publications)

Rabbit Polyclonal MTTP/MTP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

MTP, MTTP, Microsomal triglyceride transfer protein large subunit

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MTTP/MTP antibody (AB63467)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MTTP/MTP antibody (AB63467)

ab63467 staining MTTP/MTP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab63467 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Western blot - Anti-MTTP/MTP antibody (AB63467)

All lanes:

Western blot - Anti-MTTP/MTP antibody (ab63467) at 1 µg/mL

All lanes:

Liver (Human) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Predicted band size: 99 kDa

Observed band size: 99 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces (PubMed : 15897609, PubMed : 16478722, PubMed : 22236406, PubMed : 23475612, PubMed : 25108285, PubMed : 26224785, PubMed : 8876250, PubMed : 8939939). Required for the assembly and secretion of plasma lipoproteins that contain apolipoprotein B (PubMed : 16478722, PubMed : 23475612, PubMed : 26224785, PubMed : 8876250, PubMed : 8939939). May be involved in regulating cholesteryl ester biosynthesis in cells that produce lipoproteins (By similarity).

See full target information MTTP

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology 41:1567-1581 PubMed36823355

2023

Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Delilah Hendriks,Jos F Brouwers,Karien Hamer,Maarten H Geurts,Léa Luciana,Simone Massalini,Carmen López-Iglesias,Peter J Peters,Maria J Rodríguez-Colman,Susana Chuva de Sousa Lopes,Benedetta Artegiani,Hans Clevers

Bio-protocol 12: PubMed36618093

2022

Synthesis of Novel MicroRNA-30c Analogs to Reduce Apolipoprotein B Secretion in Human Hepatoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Ying Zheng,Phensinee Haruehanroengra,Pradeep Kumar Yadav,Sarah Irani,Song Mao,Ting Wang,Mahmood M Hussain,Jia Sheng

Journal of lipid research 63:100257 PubMed35931202

2022

Generation of hepatoma cell lines deficient in microsomal triglyceride transfer protein.

Applications

Unspecified application

Species

Unspecified reactive species

Narasimha Anaganti,Atrayee Chattopadhyay,John T Poirier,M Mahmood Hussain

The Journal of biological chemistry 298:101813 PubMed35278429

2022

Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pradeep Kumar Yadav,Phensinee Haruehanroengra,Sara Irani,Ting Wang,Abulaish Ansari,Jia Sheng,M Mahmood Hussain

Antiviral research 148:5-14 PubMed29074218

2017

Avasimibe: A novel hepatitis C virus inhibitor that targets the assembly of infectious viral particles.

Applications

Unspecified application

Species

Unspecified reactive species

Longbo Hu,Jinqian Li,Hua Cai,Wenxia Yao,Jing Xiao,Yi-Ping Li,Xiu Qiu,Huimin Xia,Tao Peng

Toxicology reports 3:98-104 PubMed28959527

2015

Sodium sulfite promotes the assembly and secretion of very low-density lipoprotein in HL-7702 hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jianying Bai,Peiyu Lei,Chunyan Zhao,Youping Wang,Dandan Yan,Shuyun Yang

The American journal of clinical nutrition 96:90-101 PubMed22648712

2012

Rapid alternative absorption of dietary long-chain fatty acids with upregulation of intestinal glycosylated CD36 in liver cirrhosis.

Applications

Unspecified application

Species

Human

Yasunori Yamamoto,Yoichi Hiasa,Hidehiro Murakami,Yoshio Ikeda,Hirofumi Yamanishi,Masanori Abe,Bunzo Matsuura,Morikazu Onji

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTTP/MTP antibody

Immunocytochemistry/ Immunofluorescence - Anti-MTTP/MTP antibody (AB63467)

Western blot - Anti-MTTP/MTP antibody (AB63467)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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