Anti-mu Crystallin antibody [EPR28406-56] (ab314876)

Rabbit Recombinant Monoclonal mu Crystallin antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-mu Crystallin antibody [EPR28406-56] (AB314876), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	IP	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information CRYM

Function

Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.

Alternative names

THBP, CRYM, Ketimine reductase mu-crystallin, NADP-regulated thyroid-hormone-binding protein

Recommended products

Rabbit Recombinant Monoclonal mu Crystallin antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28406-56
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Mu Crystallin also known as CRYM is a protein with a molecular weight of approximately 33 kDa. It binds to NADPH functioning as a crystallin-like structure. Mu Crystallin expresses in several tissues with notable presence in the human kidney heart and inner ear. While its original identification was in relation to vertebrate eye lens outside the lens its expression takes on additional roles that align with its involvement in thyroid hormone binding.

Biological function summary

Mu Crystallin plays a part in the regulation of thyroid hormones by acting as a carrier protein for triiodothyronine (T3). It does not belong to any known complex but shows distinction in its binding capabilities. By binding with T3 mu Crystallin modulates the availability of this hormone affecting local cellular environments and hormonal pathways. This interaction influences the normal signaling of thyroid hormones which are essential for maintaining many physiological processes.

Pathways

Mu Crystallin is involved in key processes related to thyroid hormone signaling and metabolic regulation. It fits importantly into the thyroid hormone pathway where it impacts the transportation and eventual metabolism of T3. In relation to this context mu Crystallin interacts with proteins such as thyroid hormone receptors and other binding proteins inherent in the metabolic pathways.

Associated diseases and disorders

Defects and dysregulation in mu Crystallin function connect to non-syndromic deafness and depressive disorders. Through its role in hormone regulation mu Crystallin's dysfunctions can impact neurotransmitter levels which may contribute to depressive conditions. Furthermore its relationship with other thyroid hormone-related proteins suggests implications in auditory impairments where alterations in hormone availability affect inner ear health.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: placenta (PMID:1384048), liver (PMID:1384048).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-mu Crystallin antibody [EPR28406-56] (ab314876) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: Human kidney tissue lysate at 20 µg
Lane 4: Human liver tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 33 kDa, 36 kDa
Exposure time: 15s
Western blot - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: placenta (PMID:1384048), lung (PMID:1384048).

The identity of the bands at approximately 20 kDa and 100kDa (in lane 3) are unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-mu Crystallin antibody [EPR28406-56] (ab314876) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse placenta tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat placenta tissue lysate at 20 µg
Lane 5: Rat lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa, 124 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: No staining on rat lung (PMID:1384048). The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: No staining on mouse lung. The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: Nearly no staining on human placenta (PMID:1384048). The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID:31213993). The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling mu Crystallin with ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-mu Crystallin antibody [EPR28406-56] (ab314876)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-mu Crystallin antibody [EPR28406-56] (ab314876) at 1/1000 dilution
All lanes: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa
Exposure time: 48s