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Rabbit Recombinant Monoclonal mu Crystallin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (AB314877), expandable thumbnail
  • Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (AB314877), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (AB314877), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (AB314877), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (AB314877), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal mu Crystallin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28406-56

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab314877 is the carrier-free version of Anti-mu Crystallin antibody [EPR28406-56] ab314876.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Mu Crystallin also known as CRYM is a protein with a molecular weight of approximately 33 kDa. It binds to NADPH functioning as a crystallin-like structure. Mu Crystallin expresses in several tissues with notable presence in the human kidney heart and inner ear. While its original identification was in relation to vertebrate eye lens outside the lens its expression takes on additional roles that align with its involvement in thyroid hormone binding.

Biological function summary

Mu Crystallin plays a part in the regulation of thyroid hormones by acting as a carrier protein for triiodothyronine (T3). It does not belong to any known complex but shows distinction in its binding capabilities. By binding with T3 mu Crystallin modulates the availability of this hormone affecting local cellular environments and hormonal pathways. This interaction influences the normal signaling of thyroid hormones which are essential for maintaining many physiological processes.

Pathways

Mu Crystallin is involved in key processes related to thyroid hormone signaling and metabolic regulation. It fits importantly into the thyroid hormone pathway where it impacts the transportation and eventual metabolism of T3. In relation to this context mu Crystallin interacts with proteins such as thyroid hormone receptors and other binding proteins inherent in the metabolic pathways.

Associated diseases and disorders

Defects and dysregulation in mu Crystallin function connect to non-syndromic deafness and depressive disorders. Through its role in hormone regulation mu Crystallin's dysfunctions can impact neurotransmitter levels which may contribute to depressive conditions. Furthermore its relationship with other thyroid hormone-related proteins suggests implications in auditory impairments where alterations in hormone availability affect inner ear health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: placenta (PMID:1384048), liver (PMID:1384048).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-mu Crystallin antibody [EPR28406-56] (Anti-mu Crystallin antibody [EPR28406-56] ab314876) at 1/1000 dilution

    Lane 1: Human placenta tissue lysate at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Lane 4: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 33 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: placenta (PMID:1384048), lung (PMID:1384048).

    The identity of the bands at approximately 20 kDa and 100kDa (in lane 3) are unknown.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-mu Crystallin antibody [EPR28406-56] (Anti-mu Crystallin antibody [EPR28406-56] ab314876) at 1/1000 dilution

    Lane 1: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 2: Mouse placenta tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Lane 4: Rat placenta tissue lysate at 20 µg

    Lane 5: Rat lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 33 kDa, 124 kDa

    Exposure time: 15s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: placenta (PMID:1384048), lung (PMID:1384048).

    The identity of the bands at approximately 20 kDa and 100kDa (in lane 3) are unknown.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: No staining on rat lung (PMID:1384048). The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: No staining on mouse lung. The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: Nearly no staining on human placenta (PMID:1384048). The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID:31213993). The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling mu Crystallin with Anti-mu Crystallin antibody [EPR28406-56] ab314876 at 1/1000 (0.502 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with Anti-mu Crystallin antibody [EPR28406-56] ab314876 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877), expandable thumbnail

    Western blot - Anti-mu Crystallin antibody [EPR28406-56] - BSA and Azide free (ab314877)

    This data was developed using Anti-mu Crystallin antibody [EPR28406-56] ab314876, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-mu Crystallin antibody [EPR28406-56] (Anti-mu Crystallin antibody [EPR28406-56] ab314876) at 1/1000 dilution

    All lanes: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 33 kDa

    Exposure time: 48s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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