AB28081

Anti-MUC1 antibody [C595 (NCRC48)]

Name: Anti-MUC1 antibody [C595 (NCRC48)] (ab28081) | Abcam
Brand: Abcam Limited
SKU: AB28081
Price: 545 USD
Availability: InStock

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(15 Publications)

Mouse Monoclonal MUC1 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF, AP, IHC-Fr and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MUC1.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [C595 (NCRC48)] (AB28081)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [C595 (NCRC48)] (AB28081)

FITC-conjugated ab28081 staining MUC1 in MCF-7 cells (green). Nuclei are counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [C595 (NCRC48)] (AB28081)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [C595 (NCRC48)] (AB28081)

ab28081 staining human normal lung. Staining is localised to the apical cell membrane.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir

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Western blot - Anti-MUC1 antibody [C595 (NCRC48)] (AB28081)

All lanes:

Western blot - Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)

All lanes:

Whole cell lysate prepared from human BT20 cells

Predicted band size: 122 kDa

Observed band size: 170 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C595 (NCRC48)

Isotype

IgG3

Carrier free

Reacts with

Human

Applications

IHC-Fr, ICC/IF, IHC-P, AP, WB, ELISA, Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human MUC1.

P15941

Epitope

ab28081 recognises the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.

Specificity

ab28081 recognises the breast cancer associated mucin encoded by the Muc-1 gene, CD227. In normal tissues expression is restricted to specialised glandular epithelial cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "AP" : {"fullname" : "Affinity Purification", "shortname":"AP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg3-monoclonal-ppv-07-isotype-control-ab91537'>ab91537</a> - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

See full target information MUC1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:651 PubMed34140633

2021

Menstrual flow as a non-invasive source of endometrial organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Tereza Cindrova-Davies,Xiaohui Zhao,Kay Elder,Carolyn J P Jones,Ashley Moffett,Graham J Burton,Margherita Y Turco

Scientific reports 9:16641 PubMed31719620

2019

Products of Chemoenzymatic Synthesis Representing MUC1 Tandem Repeat Unit with T-, ST- or STn-antigen Revealed Distinct Specificities of Anti-MUC1 Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Yayoi Yoshimura,Kaori Denda-Nagai,Yoshie Takahashi,Izuru Nagashima,Hiroki Shimizu,Toshimitsu Kishimoto,Miki Noji,Shigeyuki Shichino,Yasunori Chiba,Tatsuro Irimura

Biosensors & bioelectronics 142:111545 PubMed31376712

2019

Spectral contrast imaging method for mapping transmission surface plasmon images in metallic nanostructures.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yang Pan,Deng-Kai Yang,Chih-Yu Lai,Jui-Hong Weng,Kuang-Li Lee,Lin-Chi Chen,Chia-Fu Chou,Pei-Kuen Wei

Cancer cell international 15:110 PubMed26612978

2015

Morphological and molecular characterization of the human breast epithelial cell line M13SV1 and its tumorigenic derivatives M13SV1-R2-2 and M13SV1-R2-N1.

Applications

ICC/IF

Species

Human

Sandra Stempin,Anika Engel,Nora Winkler,Thorsten Buhrke,Alfonso Lampen

PloS one 8:e72637 PubMed24039787

2013

Epimorphin-induced MET sensitizes ovarian cancer cells to platinum.

Applications

Species

Human

Kok-Hooi Yew,Jennifer Crow,Jeff Hirst,Ziyan Pessetto,Ziyan Pressetto,Andrew K Godwin

Clinical breast cancer 13:109-18 PubMed23122537

2012

Expression of underglycosylated MUC1 antigen in cancerous and adjacent normal breast tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Subrata K Ghosh,Pamela Pantazopoulos,Zdravka Medarova,Anna Moore

International journal of cancer 132:1860-7 PubMed23015160

2012

Targeted imaging of breast tumor progression and therapeutic response in a human uMUC-1 expressing transgenic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Subrata K Ghosh,Masashi Uchida,Byunghee Yoo,Alana W Ross,Sandra J Gendler,Jianlin Gong,Anna Moore,Zdravka Medarova

Nephrology, dialysis, transplantation : official p 27:3082-90 PubMed22287659

2012

In vitro reconstitution of human kidney structures for renal cell therapy.

Applications

Unspecified application

Species

Human

Nadia K Guimaraes-Souza,Liliya M Yamaleyeva,Tamer AbouShwareb,Anthony Atala,James J Yoo

Analytical chemistry 82:10021-9 PubMed21077635

2010

Glycoblotting-assisted O-glycomics: ammonium carbamate allows for highly efficient o-glycan release from glycoproteins.

Applications

AP, WB

Species

Human, Human

Yoshiaki Miura,Kentaro Kato,Yasuhiro Takegawa,Masaki Kurogochi,Jun-Ichi Furukawa,Yasuro Shinohara,Noriko Nagahori,Maho Amano,Hiroshi Hinou,Shin-Ichiro Nishimura

Journal of clinical oncology : official journal of the American Society of Clinical Oncology 18:363-70 PubMed10637251

2000

Targeting superficial bladder cancer by the intravesical administration of copper-67-labeled anti-MUC1 mucin monoclonal antibody C595.

Applications

Unspecified application

Species

Unspecified reactive species

O D Hughes,M C Bishop,A C Perkins,M L Wastie,G Denton,M R Price,M Frier,H Denley,R Rutherford,P A Schubiger

View all publications

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