AB70475

Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

Name: Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475) | Abcam
Brand: Abcam Limited
SKU: AB70475
Price: 230 USD
Availability: InStock
Rating: 4 (1 reviews)

Recombinant
What is this?

(1 Review)

(29 Publications)

Mouse Recombinant Monoclonal MUC1 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Human samples. Cited in 29 publications.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissues labeling MUC1 with ab70475 at 1/5000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab70475 for 30 mins at room temperature and followed by mouse IgG antibody (ab125913, 1 : 1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissues labeling MUC1 with ab70475 at 1/5000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab70475 for 30 mins at room temperature and followed by mouse IgG antibody (ab125913, 1 : 1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Flow Cytometry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Flow Cytometry analysis of HCT 116 (Human colorectal carcinoma epithelial cell, Left) / MCF7 ( Human breast adenocarcinoma epithelial cell, Right) cells stained for MUC1 using ab70475 at a 1/1000 dilution (0.1 μg) (Red) compared to Mouse monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Negative control; HCT 116. (PMID : 14998492)

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

MCF7/ HCT 116 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab70475 at 1 : 100 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat antiMouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing membranous and cytoplasmic staining in MCF7 cells. Negative control : HCT 116 (PMID : 14998492)

Carrier free

Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
HRP

HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
578 PE

PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HMFG1 (aka 1.10.F3)

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

This antibody recognizes a peptide epitope (PDTR) within the VNTR region of the extracellular domain of MUC1 (PubMed ID: PMC3021526).

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AB32063

Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (AB32063)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

For a more comprehensive guide to this epitope of HMFG1 clone, we recommend the following publications;

Petrakou E et al. 1998: Epitope Mapping of Anti-MUC1 Mucin Protein Core Monoclonal Antibodies. Tumour Biology.PMID: 9422085

Verhoeyen ME et al. 1993: Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody. Immunology. PMID: 7682986

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

See full target information MUC1

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Polish journal of pathology : official journal of the Polish Society of Pathologists 75:274-286 PubMed39849871

2025

Enhancers of cancer stem cells and epithelial-mesenchymal transition: Lin28, MUC1, and lipocalin-2 as a new prognostic axis in classical invasive lobular carcinoma of the breast.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Alabiad,Raja Aljafil,Amany Shalaby,Ahmed M Yehia,Mohammed Alorini,Basma Hamed Ibrahim

Disease models & mechanisms 17: PubMed38903015

2024

Contribution of Streptococcus pseudopneumoniae and Streptococcus salivarius to vocal fold mucosal integrity and function.

Applications

Unspecified application

Species

Unspecified reactive species

Vlasta Lungova,Madhu Gowda,Jessica M Fernandez,Stephanie Bartley,Anumitha Venkatraman,Federico E Rey,Susan L Thibeault

Frontiers in oncology 13:1239957 PubMed38162496

2024

BME-free primary patient-specific organoids obtained with a one-day mimicking method to replicate the corresponding tumor for personalized treatment options.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhu,Zhechun Ding,Yini Wang,Qing Wu,Dongmei Chen,Luanhong Wang,Yuancheng Li,Yao Yao,Jiman Huang,Yun Li,Xiaojing Wang,Yanchun Lin,Tian Guan,Haoyu Zeng,Congzhu Li

Cellular and molecular life sciences : CMLS 80:57 PubMed36729271

2023

Relationships of tumor differentiation and immune infiltration in gastric cancers revealed by single-cell RNA-seq analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Jingwei Yang,Yongqu Lu,Yanpeng Ma,Yan Meng,Qingqing Li,Junpeng Gao,Zhaoyu Jiang,Limei Guo,Wei Wang,Yun Liu,Lu Wen,Miao Kai,Wei Fu,Fuchou Tang

Bio-protocol 12: PubMed36618088

2022

Isolation and Expansion of Primary Conjunctival Stem Cells (CjSCs) from Human and Rabbit Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhong,Shaochen Chen

Pathology oncology research : POR 28:1610684 PubMed36561231

2022

Negative correlation between the nuclear size and nuclear Lamina component Lamin A in intraductal papillary mucinous neoplasms of the pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Tamaki Hiroe,Shunichi Moriya,Sayaka Kobayashi,Yoshimi Nishijima,Akira Watanabe,Ken Shirabe,Hayato Ikota,Hideaki Yokoo,Masanao Saio

Cell discovery 8:92 PubMed36104333

2022

Single-cell profiling reveals molecular basis of malignant phenotypes and tumor microenvironments in small bowel adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Yang,Xin Zhou,Ji Dong,Wendong Wang,Yongqu Lu,Yuan Gao,Yu Zhang,Yunuo Mao,Junpeng Gao,Wei Wang,Qingqing Li,Shuai Gao,Lu Wen,Wei Fu,Fuchou Tang

Disease models & mechanisms 15: PubMed35770504

2022

Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Vlasta Lungova,Kristy Wendt,Susan L Thibeault

PloS one 17:e0266218 PubMed35385514

2022

Surface conjugation of antibodies improves nanoparticle uptake in bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie L Luks,Hanna Mandl,Jenna DiRito,Christina Barone,Mollie R Freedman-Weiss,Adele S Ricciardi,Gregory G Tietjen,Marie E Egan,W Mark Saltzman,David H Stitelman

Aging 14:2025-2046 PubMed35255004

2022

Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ramesh Pothuraju,Priya Pai,Sanjib Chaudhary,Jawed A Siddiqui,Jesse L Cox,Sukhwinder Kaur,Satyanarayana Rachagani,Hemant K Roy,Michael Bouvet,Surinder K Batra

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Flow Cytometry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB70475)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (29)

Product promise