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Rabbit Recombinant Monoclonal MUC1 antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MUC1.

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Images

Immunocytochemistry - Anti-MUC1 antibody [HMFG2] (AB245693), expandable thumbnail
  • Flow Cytometry - Anti-MUC1 antibody [HMFG2] (AB245693), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG2] (AB245693), expandable thumbnail
  • Western blot - Anti-MUC1 antibody [HMFG2] (AB245693), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.02% Proclin 300
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human MUC1. Database link P15941

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCFlow CytWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
4 µg/mL
Notes

Microwave.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
0.1 µg/mL
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Alternative names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

Recommended products

Rabbit Recombinant Monoclonal MUC1 antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MUC1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human MUC1. Database link P15941
Clone number
HMFG2
Purification technique
Affinity purification Protein A
Light chain type
lambda
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry - Anti-MUC1 antibody [HMFG2] (ab245693), expandable thumbnail

    Immunocytochemistry - Anti-MUC1 antibody [HMFG2] (ab245693)

    Immunocytochemical analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling MUC1 using ab245693 at 10 μg/ml for 1 hour (green), followed by Alexa Fluor® 488 secondary antibody (1 μg/ml). The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-MUC1 antibody [HMFG2] (ab245693), expandable thumbnail

    Flow Cytometry - Anti-MUC1 antibody [HMFG2] (ab245693)

    MCF7 (Human breast adenocarcinoma cell line) cells were stained with unimmunized rabbit IgG antibody (black line) or ab245693 (blue line) at a concentration of 10 μg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG2] (ab245693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG2] (ab245693)

    Paraffin-embedded human lung tissue stained for MUC1 with ab245693 at 4 μg/ml, 30 mins in immunohistochemical analysis. Antigen retreival was acheived by microwaving in citrate buffer (pH 6), followed by blocking with protein block serum-free buffer. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3′-diaminobenzidine), and counterstaining with hemotoxylin.

  • Western blot - Anti-MUC1 antibody [HMFG2] (ab245693), expandable thumbnail

    Western blot - Anti-MUC1 antibody [HMFG2] (ab245693)

    10% SDS PAGE gel.

    Detected by chemiluminescence.

    All lanes: Western blot - Anti-MUC1 antibody [HMFG2] (ab245693) at 0.1 µg/mL

    All lanes: MCF7 (Human breast adenocarcinoma cell line) cell lysate (RIPA buffer) at 35 µg

    Predicted band size: 122 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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