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AB216036

Anti-MUC1 antibody [HMPV]

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(1 Publication)

Mouse Monoclonal MUC1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MUC1 protein.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

1 Images
Western blot - Anti-MUC1 antibody [HMPV] (AB216036)
  • WB

Supplier Data

Western blot - Anti-MUC1 antibody [HMPV] (AB216036)

ab216036 recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.

All lanes:

Western blot - Anti-MUC1 antibody [HMPV] (ab216036)

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 122 kDa

Observed band size: 300 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HMPV

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Cell preparation containing MUC1 protein. The exact immunogen used to generate this antibody is proprietary information.

P15941

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>ab216036 recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.<br>Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 minute.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab216036 was purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
See full target information MUC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 218: PubMed33779682

2021

Inhibition of macrophage histone demethylase JMJD3 protects against abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Frank M Davis,Lam C Tsoi,William J Melvin,Aaron denDekker,Rachael Wasikowski,Amrita D Joshi,Sonya Wolf,Andrea T Obi,Allison C Billi,Xianying Xing,Christopher Audu,Bethany B Moore,Steven L Kunkel,Alan Daugherty,Hong S Lu,Johann E Gudjonsson,Katherine A Gallagher
View all publications
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Product promise

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