AB218464

Anti-MUC1 antibody [rMUC1/960]

Recombinant
What is this?

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(1 Publication)

Mouse Recombinant Monoclonal MUC1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MUC1 protein.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [rMUC1/960] (AB218464)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [rMUC1/960] (AB218464)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling MUC1 with ab218464 at 1 μg/mL for 30 minutes at RT.

Supplier Data

Western blot - Anti-MUC1 antibody [rMUC1/960] (AB218464)

MUC1 is a highly glycosylated protein with an apparent molecular weight of 265-400 kDa in Western blot.

All lanes:

Western blot - Anti-MUC1 antibody [rMUC1/960] (ab218464) at 2 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 122 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

rMUC1/960

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Cell preparation containing MUC1 protein. The exact immunogen used to generate this antibody is proprietary information.

P15941

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>MUC1 is a highly glycosylated protein with an apparent molecular weight of 265-400 kDa in Western blot.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

See full target information MUC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Med (New York, N.Y.) 4:554-579.e9 PubMed37572651

2023

Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Juan S Gnecco,Alexander Brown,Kira Buttrey,Clara Ives,Brittany A Goods,Lauren Baugh,Victor Hernandez-Gordillo,Megan Loring,Keith B Isaacson,Linda G Griffith

View all publications

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Product promise

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