Anti-MUC13 antibody [C14] (ab231159) is a mouse monoclonal antibody that is used to detect MUC13 in Flow Cytometry, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Flow Cytometry - Anti-MUC13 antibody [C14] (AB231159), expandable thumbnail](https://content.abcam.com/products/images/muc13-antibody-c14-ab231159--flow-cytometry-img38487.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MUC13 antibody [C14] (AB231159)")
![Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] (AB231159), expandable thumbnail](https://content.abcam.com/products/images/muc13-antibody-c14-ab231159--immunocytochemistry-immunofluorescence-img69564.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] (AB231159)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.082 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10.82 µg/mL | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
Alternative names
DRCC1, RECC, UNQ6194/PRO20221, MUC13, Mucin-13, MUC-13, Down-regulated in colon cancer 1
Recommended products
Anti-MUC13 antibody [C14] (ab231159) is a mouse monoclonal antibody that is used to detect MUC13 in Flow Cytometry, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- C14
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MUC13 antibody [C14] (ab231159) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Other related products
We have a range of other formats of antibody clone [C14] also available for your convenience:
ab231159, Carrier free - Anti-MUC13 antibody [C14] - BSA and Azide free ab255736
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-MUC13 antibody [C14] (ab231159), expandable thumbnail](https://content.abcam.com/products/images/muc13-antibody-c14-ab231159--flow-cytometry-img38487.jpg "Flow Cytometry - Anti-MUC13 antibody [C14] (ab231159)")
Flow Cytometry - Anti-MUC13 antibody [C14] (ab231159)
Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized T84 (human colon epithelial cell) cells labeling MUC13 with ab231159 at a 1.082μg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as the secondary at a 1/2000 dilution.
Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] (ab231159), expandable thumbnail](https://content.abcam.com/products/images/muc13-antibody-c14-ab231159--immunocytochemistry-immunofluorescence-img69564.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] (ab231159)")
Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] (ab231159)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling MUC13 with ab1231159 at 10.82μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LoVo cell line. The nuclear counterstain is DAPI (blue).
Counterstains:
Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary.The negative controls are as follows:
-ve control 1: ab231159 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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