Anti-MUC13 antibody [C14] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-MUC13 antibody [C14] - BSA and Azide free (AB255736)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab231159).

Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized T84 (human colon epithelial cell) cells labeling MUC13 with ab231159 at a 1.082μg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

Gated on viable cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] - BSA and Azide free (AB255736)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab231159).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling MUC13 with ab1231159 at 10.82μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LoVo cell line. The nuclear counterstain is DAPI (blue).
Counterstains :
ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker
ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary.

The negative controls are as follows :

-ve control 1 : ab231159 at 1/50 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at a 1/1000 dilution.

-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution.