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Rabbit Recombinant Monoclonal MUC13 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/40

Notes

-

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.

Alternative names

Recommended products

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Rabbit Recombinant Monoclonal MUC13 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21901

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Western blot - Anti-MUC13 antibody [EPR21901] (ab235450)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3 and 5: 3 minutes, Lane 4: 26 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 15904467). Lane 3 was developed using a high sensitivity ECL substrate.

    All lanes: Western blot - Anti-MUC13 antibody [EPR21901] (AB235450) at 1/1000 dilution

    Lane 1: HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 3: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: Human ovary cancer lysate at 10 µg

    Lane 5: LoVo (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Lanes 4 - 5: Western blot - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Predicted band size: 55 kDa

    Observed band size: 120 kDa, 35 kDa, 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in epithelial cells of the normal human colon (PMID: 17360025) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry (Intracellular) - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MUC13 antibody [EPR21901] (ab235450)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/40 (red) compared with a Isotype control details (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human gastric cancer (PMID: 15904467) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the apical surface and in the cytoplasm in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the LoVo cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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