Mouse Monoclonal MUC16 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MUC16 aa 14450 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (AB272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108538.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (AB272333)")
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [CL2782] (AB272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunocytochemistry-immunofluorescence-img33431.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [CL2782] (AB272333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (AB272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124015.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (AB272333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (AB272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32962.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (AB272333)")
Publications
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- Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 26:203-2192022Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhu Liu,Lian-Lian Hong,Jin-Sen Zheng,Zhe-Nan Ling,Zhi-Long Zhang,Ya-Nan Qi,Xin-Yu Zhang,Tian-Yu Zhu,Jiu-Li Wang,Jing Han,Xiang-Liu Chen,Qi-Ming Yu,Shi Wang,Pei Li,Zhi-Qiang LingPubMed 36450891
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human MUC16 aa 14450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WXI7
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Alternative names
CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125
Recommended products
Mouse Monoclonal MUC16 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MUC16 aa 14450 to C-terminus.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human MUC16 aa 14450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WXI7
- Clone number
CL2782
- Purification technique
Affinity purification
- Epitope
Binds to an epitope located within the peptide sequence NVQQQCPGYYQSHLD as determined by overlapping synthetic peptides.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108538.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333)
Paraffin-embedded human endometrium tissue stained for MUC16 using ab272333 at 1/500 dilution in immunohistochemical analysis.
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [CL2782] (ab272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunocytochemistry-immunofluorescence-img33431.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [CL2782] (ab272333)")
Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [CL2782] (ab272333)
Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling MUC16 using ab272333 at 10 μg/ml (Green). Cells also stained for nuclei (Blue) and microtubules (Red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124015.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333)
Paraffin-embedded human fallopian tube tissue stained for MUC16 using ab272333 at 1/500 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-cl2782-ab272333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32962.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [CL2782] (ab272333)
Paraffin-embedded human liver tissue stained for MUC16 using ab272333 at 1/500 dilution in immunohistochemical analysis.
Negative control.
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