JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251589

Anti-MUC16 antibody [CPT-R34-92-23] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal MUC16 antibody. Carrier free. Suitable for ELISA and reacts with Synthetic peptide - Human, Human samples. Cited in 1 publication.

View Alternative Names

CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125

1 Images
ELISA - Anti-MUC16 antibody [CPT-R34-92-23] - BSA and Azide free (AB251589)
  • ELISA

Supplier Data

ELISA - Anti-MUC16 antibody [CPT-R34-92-23] - BSA and Azide free (AB251589)

This data was developed using ab241328, the same antibody clone in a different buffer formulation.

100ng CPTAC-38b (2000ng/mL, 50 μL per well) was coated onto 96-wells. Serial dilutions (0, 0.01, 0.02, 0.05, 0.15, 0.44, 1.33, 4.00μg/mL) of ab241328 (50 μL) were incubated with CPTAC-38b in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing, 5μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CPT-R34-92-23

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB274402

Human CA125 ELISA Kit (MUC16)

Recombinant|SimpleStep

ELISA - Human CA125 ELISA Kit (MUC16) (AB274402)

  • 0
  • 0
Primary Antibodies

AB109185

Anti-MUC1 antibody [EPR1023]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (AB109185)

  • 0
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB196235

Anti-Mesothelin antibody [EPR19025-42]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-Mesothelin antibody [EPR19025-42] (AB196235)

  • 0
  • 0
Primary Antibodies

AB133633

Anti-CEACAM5 antibody [EPCEAR7]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPCEAR7] (AB133633)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab251589 is the carrier-free version of ab241328.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
See full target information MUC16
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genetics in medicine : official journal of the American College of Medical Genetics 24:1821-1830 PubMed35616648

2022

Cancer Risk C (CR-C), a functional genomics test is a sensitive and rapid test for germline mismatch repair deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ishraq Alim,Johnny Loke,Sarah Yam,Allyson S Templeton,Polly Newcomb,Noralane M Lindor,Rish K Pai,Mark A Jenkins,Daniel D Buchanan,Steven Gallinger,Susan Klugman,Harry Ostrer
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com