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AB110640

Anti-MUC16 antibody [EPR1020(2)]

4

(3 Reviews)

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(6 Publications)

Anti-MUC16 antibody [EPR1020(2)] (ab110640) is a rabbit monoclonal antibody detecting MUC16 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125

13 Images
Western blot - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • WB

Lab

Western blot - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MUC16 antibody [EPR1020(2)] (ab110640) at 1/20000 dilution

Lane 1:

Human uterus lysates at 15 µg

Lane 2:

OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 2353 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing negative staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing positive staining in Serous ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 at 1/100 dilution staining MUC16 in Human mucinous carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian serous papillary carcinoma tissue sections labeling MUC16 with Purified ab110640 at 1 : 1000 dilution (0.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing negative staining in Cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Unpurified ab110640 at 1/100 dilution staining MUC16 in Human serous carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MUC16 antibody [EPR1020(2)] (AB110640)
  • WB

Lab

Western blot - Anti-MUC16 antibody [EPR1020(2)] (AB110640)

Blocking and diluting buffer : 5% NFDM/TBST

According to PMID : 26918940 and PMID : 19747716, MCF7 and SK-OV-3 cells are MUC16 negative cell lines

All lanes:

Western blot - Anti-MUC16 antibody [EPR1020(2)] (ab110640) at 1/10000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates at 15 µg

Lane 3:

OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 2353 kDa

false

  • Carrier free

    Anti-MUC16 antibody [EPR1020(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1020(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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AB274402

Human CA125 ELISA Kit (MUC16)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Anti-MUC16 antibody [EPR1020(2)] (ab110640) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of MUC16?
Anti-MUC16 [EPR1020(2)] (ab110640) specifically detects a band for MUC16 (UniProt: Q8WXI7) at a molecular weight of 1519kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR1020(2)] also available for your convenience: ab110640, Carrier free - ab230497

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
See full target information MUC16
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 13:2849-2867 PubMed33833569

2021

A Novel, Personalized Drug-Screening System for Platinum-Resistant Ovarian Cancer Patients: A Preliminary Clinical Report.

Applications

Unspecified application

Species

Unspecified reactive species

Yunke Huang,Jing Xu,Ke Li,Jing Wang,Yilin Dai,Yu Kang

Journal of cellular and molecular medicine 25:3031-3040 PubMed33543559

2021

MUC16 promotes EOC proliferation by regulating GLUT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Qing Zhang,Hailing Zhang,Xiaogai Qiao,Xia Zhang,Ke Zhang,Xiaoli Gu,Lihong Wang,Jinquan Cui

Experimental and therapeutic medicine 21:163 PubMed33456530

2021

Inflammatory signals induce MUC16 expression in ovarian cancer cells via NF-κB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Li Li,Ning Luo,Qi Liu,Li Liu,Dandan Chen,Zhongping Cheng,Xiaowei Xi

Molecular oncology 14:2436-2454 PubMed32797726

2020

In vivo anti-V-ATPase antibody treatment delays ovarian tumor growth by increasing antitumor immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Arpita Kulshrestha,Gajendra K Katara,Safaa A Ibrahim,Valerie E Riehl,Sylvia Schneiderman,Mahmood Bilal,Alexandria N Young,Shayna Levine,Sara Fleetwood,James Dolan,Alice Gilman-Sachs,Kenneth D Beaman

The European respiratory journal 51: PubMed29371381

2018

Inhaled diesel exhaust alters the allergen-induced bronchial secretome in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Neeloffer Mookherjee,Hadeesha Piyadasa,Min Hyung Ryu,Christopher Francis Rider,Peyman Ezzati,Victor Spicer,Christopher Carlsten

Experimental cell research 360:236-242 PubMed28899659

2017

BRG1 promotes VEGF-A expression and angiogenesis in human colorectal cancer cells.

Applications

WB

Species

Unspecified reactive species

Jingqin Lan,Haijie Li,Xuelai Luo,Junbo Hu,Guihua Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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