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AB134093

Anti-MUC16 antibody [EPSISR23]

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(6 Publications)

Rabbit Recombinant Monoclonal MUC16 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (AB134093)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (AB134093)

Immunocytochemistry analysis of NIH : OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified ab134093 at 1 : 50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-MUC16 antibody [EPSISR23] (AB134093)
  • WB

Lab

Western blot - Anti-MUC16 antibody [EPSISR23] (AB134093)

Blocking and dilution buffer : 5% NFDM/TBST.

Image produced using the purified version.

All lanes:

Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093) at 1/10000 dilution

All lanes:

NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 2353 kDa

Observed band size: 2353 kDa

false

  • Carrier free

    Anti-MUC16 antibody [EPSISR23] - BSA and Azide free

  • 660 APC

    APC Anti-MUC16 antibody [EPSISR23]

  • 578 PE

    PE Anti-MUC16 antibody [EPSISR23]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-MUC16 antibody [EPSISR23]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MUC16 antibody [EPSISR23]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MUC16 antibody [EPSISR23]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-MUC16 antibody [EPSISR23]

  • Carrier free

    Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR23

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>For unpurified use at 1/100 - 1/250 dilution.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p>For unpurified use at 1/10000 - 1/50000 dilution.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
See full target information MUC16
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:571 PubMed39112478

2024

Single-cell transcriptome profiles the heterogeneity of tumor cells and microenvironments for different pathological endometrial cancer and identifies specific sensitive drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Ren,Lingfang Wang,Yuyouye Wang,Jiaxuan Wang,Yuanpei Wang,Xiaole Song,Gong Zhang,Fangfang Nie,Shitong Lin

Current eye research 47:1106-1115 PubMed35577415

2022

Human Oral Mucosal Fibroblasts from Limbal Stem Cell Deficient Patients as an Autologous Feeder Layer for Epithelial Cell Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R O'Callaghan,Alex J Shortt,Mark P Lewis,Julie T Daniels

Advanced biosystems 4:e1900265 PubMed32515079

2020

Oral Mucosa Tissue Equivalents for the Treatment of Limbal Stem Cell Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R O'Callaghan,Marc A Dziasko,Radhika Sheth-Shah,Mark P Lewis,Julie T Daniels

Sensors (Basel, Switzerland) 20: PubMed31941128

2020

Smartphone Biosensor System with Multi-Testing Unit Based on Localized Surface Plasmon Resonance Integrated with Microfluidics Chip.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Fan,Zhaoxin Geng,Weihao Fang,Xiaoqing Lv,Yue Su,Shicai Wang,Hongda Chen

Regenerative medicine 11:529-43 PubMed27513189

2016

Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R O'Callaghan,Louise Morgan,Julie T Daniels,Mark P Lewis

International journal of molecular medicine 33:1355-63 PubMed24626634

2014

Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiji Yamada,Tamotsu Nishida,Hideki Horibe,Mitsutoshi Oguri,Kimihiko Kato,Motoji Sawabe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com