Rabbit Recombinant Monoclonal MUC16 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 4 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (AB134093), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-ab134093--immunocytochemistry-immunofluorescence-img169822.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (AB134093)")
![Western blot - Anti-MUC16 antibody [EPSISR23] (AB134093), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-ab134093--western-blot-img165749.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MUC16 antibody [EPSISR23] (AB134093)")
Publications
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- Current eye research 47:1106-11152022Human Oral Mucosal Fibroblasts from Limbal Stem Cell Deficient Patients as an Autologous Feeder Layer for Epithelial Cell Culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna R O'Callaghan,Alex J Shortt,Mark P Lewis,Julie T DanielsPubMed 35577415
- Advanced biosystems 4:e19002652020Oral Mucosa Tissue Equivalents for the Treatment of Limbal Stem Cell Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna R O'Callaghan,Marc A Dziasko,Radhika Sheth-Shah,Mark P Lewis,Julie T DanielsPubMed 32515079
- Sensors (Basel, Switzerland) 20:2020Smartphone Biosensor System with Multi-Testing Unit Based on Localized Surface Plasmon Resonance Integrated with Microfluidics Chip.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyuan Fan et. al.PubMed 31941128
- Regenerative medicine 11:529-432016Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna R O'Callaghan et. al.PubMed 27513189
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/100 - 1/250 dilution. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes For unpurified use at 1/10000 - 1/50000 dilution. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Alternative names
CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125
Recommended products
Rabbit Recombinant Monoclonal MUC16 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPSISR23
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (ab134093), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-ab134093--immunocytochemistry-immunofluorescence-img169822.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (ab134093)")
Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] (ab134093)
Immunocytochemistry analysis of NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified ab134093 at 1:50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-ab134093--western-blot-img165749.jpg "Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093)")
Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093)
Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
All lanes: Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093) at 1/10000 dilution
All lanes: NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 2353 kDa
Observed band size: 2353 kDa
Downloads
Product protocols
- Visit the general protocols
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