Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (AB215381)

This data was developed using ab134093, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NIH : OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified ab134093 at 1 : 50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Lab

Western blot - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (AB215381)

This data was developed using ab134093, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Image produced using the purified version.

All lanes:

Western blot - Anti-MUC16 antibody [EPSISR23] (<a href='/en-us/products/primary-antibodies/muc16-antibody-epsisr23-ab134093'>ab134093</a>) at 1/10000 dilution

All lanes:

NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 2353 kDa

Observed band size: 2353 kDa

false