Rabbit Recombinant Monoclonal MUC16 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (AB215381), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-low-endotoxin-azide-free-ab215381--immunocytochemistry-immunofluorescence-img169822.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (AB215381)")
![Western blot - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (AB215381), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-low-endotoxin-azide-free-ab215381--western-blot-img165749.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (AB215381)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
3 products for Alternative Version
Target data
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Alternative names
CA125, MUC16, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, CA-125
Recommended products
Rabbit Recombinant Monoclonal MUC16 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPSISR23
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab215381 is the carrier-free version of Anti-MUC16 antibody [EPSISR23] ab134093.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-low-endotoxin-azide-free-ab215381--immunocytochemistry-immunofluorescence-img169822.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381)")
Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381)
This data was developed using Anti-MUC16 antibody [EPSISR23] ab134093, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified Anti-MUC16 antibody [EPSISR23] ab134093 at 1:50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Western blot - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381), expandable thumbnail](https://content.abcam.com/products/images/muc16-antibody-epsisr23-low-endotoxin-azide-free-ab215381--western-blot-img165749.jpg "Western blot - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381)")
Western blot - Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381)
This data was developed using Anti-MUC16 antibody [EPSISR23] ab134093, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
All lanes: Western blot - Anti-MUC16 antibody [EPSISR23] (Anti-MUC16 antibody [EPSISR23] ab134093) at 1/10000 dilution
All lanes: NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 2353 kDa
Observed band size: 2353 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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